Diversity and Evolution of the Phenazine Biosynthesis Pathway
Diversity and Evolution of the Phenazine Biosynthesis Pathway
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DOI：
10.1128/aem.02009-09
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Thomashow, Linda S.
中科院分区：
文献类型：
--
作者：
Mavrodi, Dmitri V.;Peever, Tobin L.;Thomashow, Linda S.
非那嗪是细菌来源的多用途次生代谢物，在植物病原体的生物控制中起作用，并有助于生产菌株的生态适应性和致病性。在这项研究中，我们收集了94株具有不同地理、环境和临床起源的菌株，研究了假单胞菌、伯克霍尔德菌、乳杆菌、短杆菌和链霉菌属成员的非那嗪基因的分布和进化。我们的研究结果证实了苯那嗪产生者的多样性，并揭示了它们中的大多数似乎是土壤栖息和/或植物相关物种。基因组分析和从关键的非那嗪生物合成（phzF）和整理(rrs, recA, rpoB, atpD，和gyrB)基因揭示了非那嗪基因的进化和传播机制，从假单胞菌的保守到伯克霍尔德氏菌和Pectobacterium的水平基因转移。从谷物根际提取的DNA中筛选phzF的存在，其序列与华盛顿州中部商业农场中多种非那嗪生产商群体的存在一致。这提供了美国土壤富含本土产非那嗪细菌的第一个证据。
Phenazines are versatile secondary metabolites of bacterial origin that function in biological control of plant pathogens and contribute to the ecological fitness and pathogenicity of the producing strains. In this study, we employed a collection of 94 strains having various geographic, environmental, and clinical origins to study the distribution and evolution of phenazine genes in members of the genera Pseudomonas, Burkholderia, Pectobacterium, Brevibacterium, and Streptomyces. Our results confirmed the diversity of phenazine producers and revealed that most of them appear to be soil-dwelling and/or plant-associated species. Genome analyses and comparisons of phylogenies inferred from sequences of the key phenazine biosynthesis (phzF) and housekeeping (rrs, recA, rpoB, atpD, and gyrB) genes revealed that the evolution and dispersal of phenazine genes are driven by mechanisms ranging from conservation in Pseudomonas spp. to horizontal gene transfer in Burkholderia spp. and Pectobacterium spp. DNA extracted from cereal crop rhizospheres and screened for the presence of phzF contained sequences consistent with the presence of a diverse population of phenazine producers in commercial farm fields located in central Washington state, which provided the first evidence of United States soils enriched in indigenous phenazine-producing bacteria.