A chemiluminescence assay for L-histidine based on controlled DNAzyme catalytic reactions on magnetic microparticles
A chemiluminescence assay for L-histidine based on controlled DNAzyme catalytic reactions on magnetic microparticles
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基于磁性微粒上受控 DNAzyme 催化反应的 L-组氨酸化学发光测定
DOI：
10.1007/s00604-014-1359-6
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发表时间：
2015-02-01
影响因子：
5.7
通讯作者：
Bai, Li
中科院分区：
文献类型：
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作者：
Hun, Xu;Xu, Yaqiong;Bai, Li
我们描述了一种l -组氨酸的化学发光（CL）测定方法，该方法基于使用DNAzyme共价固定在1.5 μ m大小的磁珠上。在添加用CL试剂标记的底物时，DNAzyme和底物通过每个双相的变构协同稳定形成稳定的双相。如果将l -组氨酸加入到该体系中，则通过催化反应发生底物的自裂解，并导致形成两个片段，这些片段与珠子分离。去除磁珠后，标记片段可以用CL检测，其强度在1.0 ~ 1000 nM范围内与l -组氨酸浓度线性相关。在50 nM水平（n = 9），检测限为0.3 nM， RSD为3.4%。该方法已成功应用于加标人血清样品中l -组氨酸的测定，有望成为基于dnazyme的小有机分子和金属离子CL检测的通用平台。
We describe a chemiluminescence (CL) assay for L-histidine that is based on the use of DNAzyme covalently immobilized on 1.5-mu m sized magnetic beads. On addition of a substrate labeled with a CL reagent, the DNAzyme and substrate form a stable duplex by allosteric synergetic stabilization of each duplex. If L-histidine is added to this system, self-cleavage of the substrate occurs through catalytic reaction and results in the formation of two fragments which dissociate from the beads. After removal of the magnetic beads, the labeled fragments can be detected by CL whose intensity is linearly related to the concentration of L-histidine in the 1.0 to 1,000 nM range. The detection limit is 0.3 nM, and the RSD is 3.4 % at a 50 nM level (n = 9). The method has been successfully applied to the determination of L-histidine in spiked human serum samples and holds promise as a widely applicable general platform for DNAzyme-based CL detection of small organic molecules and of metal ions.