Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
复制标题
利用 RNA 干扰沉默气味结合蛋白基因 OBP3 减少番茄褪绿病毒的病毒传播
DOI：
10.3390/ijms20204969
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发表时间：
2019-10-02
影响因子：
5.6
通讯作者：
Liu, Yong
中科院分区：
文献类型：
--
作者：
Shi, Xiao-Bin;Wang, Xue-Zhong;Liu, Yong
番茄萎黄病毒（ToCV）在世界范围内广泛传播，严重影响番茄生产。ToCV由烟粉虱半持久性传播（半翅目：烟粉虱科）。目前，人们正在研究昆虫嗅觉，以开发新的害虫防治技术，有效防治烟粉虱和白蝇传播的病毒疾病。尽管目前的研究努力，但在植物病毒影响下，气味结合蛋白（OBPs）在昆虫偏好中的作用尚未发表报告。我们之前的研究表明，在获得病毒48 h后，毒性烟粉虱偏爱健康植株。在本研究中，我们确定了OBPs对ToCV和白蝇寄主偏好相互作用的影响。结果表明，随着获取时间的增加，OBP基因表达量发生了不同的变化，OBP3基因表达量呈现先上升后下降的趋势，并在48 h时达到最大值，说明OBP3可能参与了毒粉虱对健康植物的寄主偏好。当RNA干扰（RNAi）技术敲除OBP3基因的表达时，地中海病毒（MED）没有表现出偏好，ToCV的传播率降低了83.3%。结果表明，OBP3基因参与了毒力强的MED对植物挥发物的检测，为明确烟粉虱传播ToCV的机制提供了理论依据和技术支持，并为控制该植物病毒及其载体提供了新的途径。
Tomato chlorosis virus (ToCV) is widespread, seriously impacting tomato production throughout the world. ToCV is semi-persistently transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Currently, insect olfaction is being studied to develop novel pest control technologies to effectively control B. tabaci and whitefly-borne virus diseases. Despite current research efforts, no report has been published on the role of odorant-binding proteins (OBPs) in insect preference under the influence of plant virus. Our previous research showed that viruliferous B. tabaci preferred healthy plants at 48 h after virus acquisition. In this study, we determined the effect of OBPs on the host preference interactions of ToCV and whiteflies. Our results show that with the increase in acquisition time, the OBP gene expressions changed differently, and the OBP3 gene expression showed a trend of first rising and then falling, and reached the maximum at 48 h. These results indicate that OBP3 may participate in the host preference of viruliferous whiteflies to healthy plants. When the expression of the OBP3 gene was knocked down by an RNA interference (RNAi) technique, viruliferous Mediterranean (MED) showed no preference and the ToCV transmission rate was reduced by 83.3%. We conclude that OBP3 is involved in the detection of plant volatiles by viruliferous MED. Our results provide a theoretical basis and technical support for clarifying the transmission mechanism of ToCV by B. tabaci and could provide new avenues for controlling this plant virus and its vectors.