Dataset on the formation of Thioredoxin interacting protein (Txnip) containing redox sensitive high molecular weight nucleoprotein complexes.
Dataset on the formation of Thioredoxin interacting protein (Txnip) containing redox sensitive high molecular weight nucleoprotein complexes.
复制标题
关于包含氧化还原敏感的高分子量核蛋白复合物的硫氧还蛋白相互作用蛋白 (Txnip) 形成的数据集。
DOI：
10.1016/j.dib.2019.104893
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发表时间：
2019
期刊：
影响因子：
1.2
通讯作者：
Masutani H
中科院分区：
文献类型：
--
作者：
Hirata CL;Ito S;Masutani H
该数据集是对参考文献[1]提交的研究的补充。从高分子量复合物中提取RNA，该复合物通过对用F-HA-Txnip-V5-His或对照载体稳定转染的HEK 293 Tet-on细胞进行100 kDa过滤来制备。用1 μg/mL多西环素刺激细胞24小时，然后用100 μM 4-硫尿苷（4sU）、20 mM葡萄糖和1 μM硼替佐米过夜刺激14小时。通过RNA-seq分析与对照细胞相比从Txnip过表达细胞提取的RNA。显示了差异表达的mRNA、长非编码RNA（lncRNA）和不确定编码潜力的转录物（TUCP）。这些差异表达的RNA的基因本体和KEGG富集。
This dataset is supplementary to the submitted research by Ref. [1]. RNAs were extracted from high molecular weight complexes, prepared with 100 kDa filtration of HEK293 Tet-on cells stably transfected with either F-HA-Txnip-V5-His or control vector. Cells were stimulated with 1 μg/mL doxycycline for 24 h, followed by overnight stimulation with 100 μM 4-thiouridine (4sU), 20 mM glucose, and 1 μM bortezomib for 14h. The extracted RNAs from Txnip overexpressing cells compared with control cells was analyzed by RNA-seq. Differentially expressed mRNAs, long noncoding RNAs (lncRNA) and transcripts of uncertain coding potential (TUCPs) are shown. Gene ontology and KEGG enrichment of these differential expressed RNAs is presented.