Noninvasive functional optical spectroscopy of human breast tissue
Noninvasive functional optical spectroscopy of human breast tissue
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DOI：
10.1073/pnas.071511098
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发表时间：
2001-04-10
影响因子：
11.1
通讯作者：
Tromberg, B
中科院分区：
文献类型：
--
作者：
Shah, N;Cerussi, A;Tromberg, B
近红外漫反射光谱和漫反射光学成像是有前景的方法，最终可能增强或取代现有的乳腺癌筛查和诊断技术。这些技术基于对健康组织和患病组织之间的光学和功能对比的高度灵敏的定量测量。在这项研究中，我们研究了外源激素、衰老和月经周期波动引起的乳腺生理变化是否会导致乳腺组织光学对比度的显着变化。使用了一种非侵入性定量漫反射光谱技术，即频域光子迁移。使用手持式探头对 14 名志愿者受试者进行了测量。根据频域光子迁移数据计算固有组织吸收和减少散射参数。波长依赖性吸收（674、803、849 和 956 nm）用于测定氧合血红蛋白、脱氧血红蛋白、总血红蛋白、组织血红蛋白氧饱和度和体水含量的组织浓度。结果显示绝经状态之间的光学特性存在显着且显着的差异。与绝经后受试者相比，每个波长的平均绝经前固有组织吸收和减少散射值分别高 2.5 至 3 倍和 16-28%。使用激素替代疗法的女性的吸收和散射特性介于绝经前和绝经后人群之间。绝经后、激素替代治疗和绝经前受试者的生理特性显示，相对于纯水，平均总血红蛋白（7.0 μM、11.8 μM 和 19.2 μM）和水浓度（10.9%、15.3% 和 27.3%）存在差异。由于其独特的定量信息内容，漫射光学方法可能在乳腺诊断和提高我们对乳腺疾病的了解中发挥重要作用。
Near infrared diffuse optical spectroscopy and diffuse optical imaging are promising methods that eventually may enhance or replace existing technologies for breast cancer screening and diagnosis. These techniques are based on highly sensitive, quantitative measurements of optical and functional contrast between healthy and diseased tissue. In this study, we examine whether changes in breast physiology caused by exogenous hormones, aging, and fluctuations during the menstrual cycle result in significant alterations in breast tissue optical contrast. A noninvasive quantitative diffuse optical spectroscopy technique, frequency-domain photon migration, was used. Measurements were performed on 14 volunteer subjects by using a hand-held probe. Intrinsic tissue absorption and reduced scattering parameters were calculated from frequency-domain photon migration data. Wavelength-dependent absorption (at 674, 803, 849, and 956 nm) was used to determine tissue concentration of oxyhemoglobin, deoxyhemoglobin, total hemoglobin, tissue hemoglobin oxygen saturation, and bulk water content. Results show significant and dramatic differences in optical properties between menopausal states. Average premenopausal intrinsic tissue absorption and reduced scattering values at each wavelength are 2.5- to 3-fold higher and 16-28% greater, respectively, than absorption and scattering for postmenopausal subjects. Absorption and scattering properties for women using hormone replacement therapy are intermediate between premenopausal and postmenopausal populations. Physiological properties show differences in mean total hemoglobin (7.0 muM, 11.8 muM, and 19.2 muM) and water concentration relative to pure water (10.9%, 15.3%, and 27.3%) for postmenopausal, hormone replacement therapy, and premenopausal subjects, respectively. Because of their unique, quantitative information content, diffuse optical methods may play an important role in breast diagnostics and improving our understanding of breast disease.