The interaction of indocyanine green with human retinal pigment epithelium
The interaction of indocyanine green with human retinal pigment epithelium
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DOI：
10.1167/iovs.04-0825
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发表时间：
2005-04-01
影响因子：
4.4
通讯作者：
Billson, F
中科院分区：
文献类型：
--
作者：
Chang, AA;Zhu, MD;Billson, F
目的。本研究旨在检测人RPE细胞与吲哚青绿(ICG)的体外相互作用。ICG和RPE之间的相互作用在临床ICG血管造影的解释中可能具有临床意义。用红外荧光显微镜检测RPE细胞ICG荧光。对显示ICG红外荧光的细胞比例进行量化。将不同的RPE细胞群体分别与哇巴因孵育24小时和72小时，然后加入ICG，以检测其对ICG摄取的影响。台盼蓝拒染法检测哇巴因对RPE细胞活力的影响。结果：ICG孵育的正常人RPE细胞具有较强的红外荧光。哇巴因在与ICG孵育前24小时对细胞活力影响不大，但显著降低细胞ICG荧光。相反，哇巴因暴露72小时会降低细胞活力，增加细胞ICG荧光。结论：培养的人RPE细胞摄取ICG染料。RPE细胞对ICG的摄取可能涉及主动转运，因为与哇巴因孵育24小时的细胞显示细胞活力没有下降，但红外荧光减弱。在更长时间接触哇巴因后，ICG被细胞摄取的矛盾增加可能是由于ICG在受损的细胞膜中移动所致。RPE摄取ICG引起的荧光具有临床意义，因为它对ICG血管造影观察到的荧光模式有贡献。
PURPOSE. This study sought to examine the in vitro interaction of human RPE cells with indocyanine green (ICG). The interaction between ICG and the RPE may have clinical relevance in the interpretation of clinical ICG angiography.METHOD. Cultured primary human RPE cells were incubated with ICG. Infrared fluorescence microscopy was used to detect RPE cell ICG fluorescence. The proportions of cells exhibiting ICG infrared fluorescence were quantified. Separate RPE cell populations were incubated with ouabain for 24 and 72 hours, respectively, before addition of the ICG to examine its effect on the uptake of ICG. The effect of ouabain on cell viability was assessed with trypan blue exclusion.RESULTS. Normal human RPE cells incubated with ICG exhibited strong infrared fluorescence. Exposure to ouabain for 24 hours before incubation with ICG had little effect on cell viability but significantly reduced cellular ICG fluorescence. In contrast, exposure to ouabain for 72 hours reduced cell viability and increased cellular ICG fluorescence.CONCLUSIONS. Cultured human RPE cells take up ICG dye. ICG uptake by RPE cells may involve active transport, as cells incubated with ouabain for 24 hours showed no reduction in cell viability but exhibited reduced infrared fluorescence. The paradoxical increased uptake of ICG into the cells after more prolonged exposure to ouabain may be due to ICG's movement through the damaged cell membrane. Fluorescence due to ICG uptake by RPE has clinical relevance in that it contributes to the fluorescence patterns observed in ICG angiography.