Clinocopathologic significance of laminin-5 gamma2 chain expression in squamous cell carcinoma of the tongue: immunohistochemical analysis of 67 lesions.
Clinocopathologic significance of laminin-5 gamma2 chain expression in squamous cell carcinoma of the tongue: immunohistochemical analysis of 67 lesions.
复制标题
舌鳞状细胞癌中层粘连蛋白 5 γ2 链表达的临床病理学意义：67 个病变的免疫组织化学分析。
作者：
Yukiko Ono;Yukihiro Nakanishi;Y. Ino;Toshiro Niki;T. Yamada;K. Yoshimura;M. Saikawa;Tamio Nakajima;Setsuo Hirohashi
背景
层粘连蛋白-5 γ 2链在肿瘤侵袭和组织重塑期间的细胞迁移中起重要作用。
方法
采用免疫组化方法检测67例Ⅱ、Ⅲ、Ⅳ A、B期舌鳞癌（不包括远处转移者）中层粘连蛋白5 γ 2链的表达，以确定其与各肿瘤临床病理特征的关系。主要染色模式分类如下：A，很少或没有肿瘤细胞呈阳性; B，部分肿瘤巢周边呈阳性; C，肿瘤巢周边呈周向阳性;或D，几乎所有肿瘤细胞均呈阳性。
结果
层粘连蛋白5 γ 2链在肿瘤细胞胞浆中表达。在67例肿瘤中，分别有6例（9%）、31例（46%）、19例（28%）和11例（17%）显示染色模式A、B、C和D。随着染色模式A到D的进展，免疫阳性肿瘤细胞的数量显著增加（P<0.0001），并且肿瘤组织学显示显著更多的浸润性生长（P<0.0001）和更差的分化（P = 0.0021）。此外，对患者生存的单变量（P = 0.0019）和多变量（P = 0.0003;风险比= 3.132）分析显示，随着染色模式A向D的进展，预后明显变差。
结论
层粘连蛋白-5 γ 2链免疫反应性的增加可能反映了癌细胞的高侵袭潜力，是舌鳞状细胞癌患者预后不良的一个指标。
BACKGROUND
The laminin-5 gamma2 chain plays an important role in cell migration during tumor invasion and tissue remodeling.
METHODS
Laminin-5 gamma2 chain expression in squamous cell carcinomas of the tongue in 67 patients with Stage II, III, or IVA,B (excluding the cases with distant metastasis) was examined immunohistochemically to determine its associations with the clinicopathologic features of each tumor. The predominant staining patterns were categorized as follows: A, few or no tumor cells were positive; B, part of the tumor nest periphery was positive; C, the tumor nest periphery was circumferentially positive; or D, almost all the tumor cells were positive.
RESULTS
Laminin-5 gamma2 chain expression was observed clearly in tumor cell cytoplasm. Of the 67 tumors examined, 6 (9%), 31 (46%), 19 (28%), and 11 (17%) showed staining patterns A, B, C, and D, respectively. With progression from staining pattern A to D, the number of immunopositive tumor cells increased significantly (P<0.0001), and the tumor histology showed significantly more infiltrative growth (P<0.0001) and poorer differentiation (P = 0.0021). Furthermore, both univariate (P = 0.0019) and multivariate (P = 0.0003; hazard ratio = 3.132) analysis of the patients' survival revealed that the prognosis became significantly poorer with progression from staining pattern A to D.
CONCLUSIONS
Increased laminin-5 gamma2 chain immunoreactivity, which may reflect a high invasive potential of cancer cells, is a factor indicative of a poor prognosis for patients with squamous cell carcinoma of the tongue.