STRUCTURAL OF SHELL AND OUTER MEMBRANES IN ENCYSTED ARTEMIA SALINA EMBRYOS DURING CRYPTOBIOSIS AND DEVELOPMENT
STRUCTURAL OF SHELL AND OUTER MEMBRANES IN ENCYSTED ARTEMIA SALINA EMBRYOS DURING CRYPTOBIOSIS AND DEVELOPMENT
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DOI：
10.1016/s0022-5320(67)90285-7
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
AFZELIUS, BA
中科院分区：
文献类型：
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作者：
MORRIS, JE;AFZELIUS, BA
隐生和发育中的卤虫包囊的切片，A。盐藻，戊二醛和四氧化锇固定电镜。壳类似于昆虫的蛋壳，有一个次氯酸盐可溶的绒毛膜和一个纤维状的、抗次氯酸盐的角质层。在绒毛膜和角质层之间有一层薄而复杂的膜，它能抵抗次氯酸盐溶液，也不能渗透铅离子。胚胎外的表皮下空间通常含有许多小颗粒的隐生囊肿。这些颗粒在发育过程中消失，并被孵化膜所取代。从卵囊释放后，至少有一些包囊被细胞膜覆盖。在空气干燥（即，进入隐生状态），这一外膜被破坏，随后的湿润重新启动了包囊原肠胚的发育。
Slices of cryptobiotic and developing cysts of the brine shrimp, A. salina, were fixed in glutaraldehyde and osmium tetroxide for electron microscopy. The shell was similar to insect egg shells in having a hypochlorite-soluble chorion and a fibrous, hypochlorite-resistant cuticle. Between the chorion and cuticle was a thin but complex membrane that resisted solution in hypochlorite and was impermeable to lead ions. The extra-embryonic, subcuticular space usually contained many small particles in the cryptobiotic cyst. The particles disappeared during development and were replaced by the hatching membrane. After being released from the brood sac, at least some cysts are covered by a cellular membrane. Upon air-drying (i.e., entry into cryptobiosis) this outer membrane is destroyed, and subsequent wetting reinitiates development in the encysted gastrula.