Spectroscopic detection of endovesiculation by large unilamellar phosphatidylcholine vesicles: effects of chlorpromazine, dibucaine, and safingol.
Spectroscopic detection of endovesiculation by large unilamellar phosphatidylcholine vesicles: effects of chlorpromazine, dibucaine, and safingol.
复制标题
大单层磷脂酰胆碱囊泡内囊泡的光谱检测：氯丙嗪、地布卡因和 safingol 的作用。
DOI：
10.1016/s0968-0896(99)00076-0
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发表时间：
1999
影响因子：
3.5
通讯作者：
Matile,S
中科院分区：
文献类型：
--
作者：
Tedesco,MM;Matile,S
描述了由阳离子两亲体诱导的大单层囊泡（LUVs）的内泡形成。最近一项通过测定同时内服水溶性荧光染料HPTS的量和外用猝灭剂来监测巨噬细胞吞噬囊泡的方法适用于luv （Daleke， D. L.; Hong， K.; papahadjoopoulos， D. Biochim）。Biophys。[act1990, 1024, 352]。与二布卡因和沙芬酚相比，局麻药氯丙嗪（CPZ）是luv最有效的hpts内化诱导剂。利用包埋HPTS的luv进行的对照实验表明，所观察到的染料内化并非源于瞬态裂解。超过CPZ临界胶束浓度的活性的强烈增加表明CPZ胶束对内囊化的重要性。与直径为176nm的luv相比，直径为68nm的luv诱导cpz诱导的hpts内化效率明显较低，进一步降低了胶束/双层融合机制的可能性，并支持直径小至68nm的luv存在“拉链型”内泡形成。
Endovesiculation by large unilamellar vesicles (LUVs) induced by cationic amphiphiles is described in this work. A recent procedure to monitor phagocytosis of vesicles by macrophages by determining the amount of the simultaneously internalized water-soluble fluorescent dye HPTS with external quencher was adapted to LUVs (Daleke, D. L.; Hong, K.; Papahadjopoulos, D. Biochim. Biophys. Acta1990, 1024, 352). Compared to dibucaine and safingol, the local anesthetic chlorpromazine (CPZ) was found to be the most efficient inducer of HPTS-internalization by LUVs. Control experiments using LUVs with entrapped HPTS indicated that the observed dye-internalization does not originate from transient lysis. A strong increase in activity above the critical micelle concentration of CPZ implies the importance of CPZ-micelles for endovesiculation. The significantly less efficient CPZ-induced HPTS-internalization by LUVs with 68nm compared to 176nm diameter further diminishes the likelihood of a micelle/bilayer fusion mechanism and supports the presence of ‘zipper-type’ endovesiculation by LUVs with diameters as small as 68nm.