Identification of proteins of Pro pionibacterium acnes for use as vaccine candidates to prevent infection by the pig pathogen Actinobacillus pleuropneumoniae
Identification of proteins of Pro pionibacterium acnes for use as vaccine candidates to prevent infection by the pig pathogen Actinobacillus pleuropneumoniae
复制标题
鉴定痤疮丙酸杆菌的蛋白质，用作候选疫苗以预防猪病原体胸膜肺炎放线杆菌的感染。
DOI：
10.1016/j.vaccine.2013.08.054
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发表时间：
2013-10-25
期刊：
影响因子：
5.5
通讯作者：
Lei, Liancheng
中科院分区：
文献类型：
--
作者：
Li, Linxi;Sun, Changjiang;Lei, Liancheng
胸膜肺炎放线杆菌是引起养猪业严重发病率和死亡率的急性和慢性胸膜肺炎的病原体。需要能够预防所有血清型和防止定植的新的改良疫苗。在之前的研究中，我们发现痤疮丙酸杆菌的全细胞可以保护猪免受1型和5型胸膜肺炎的侵袭，因此，这是一种有前景的异源疫苗的基础。本研究的目的是确定痤疮假单胞菌对胸膜肺炎弧菌感染具有保护作用的蛋白抗原。用双向凝胶电泳法和免疫印迹法鉴定了6种能被抗胸膜肺炎弧菌血清识别的痤疮杆菌蛋白抗原。重组的所有痤疮假单胞菌蛋白对A有部分保护作用(10-80%)。小鼠挑战模型中的胸膜肺炎血清1型和/或5型感染。重组痤疮杆菌单链DNA结合蛋白的保护率最高(80%为1型，60%为5型)。在一定程度上，对胸膜肺炎弧菌感染的保护作用可能是通过存在于痤疮螺旋体单链DNA结合蛋白中的小肽序列来介导的，这些小肽序列与胸膜肺炎弧菌特异的RTX毒素ApxIV和锌结合蛋白ZnuA中的那些存在交叉反应。提示痤疮巴氏杆菌可能是一种对不同血清型胸膜肺炎弧菌有保护作用的疫苗。(C)2013爱思唯尔有限公司。保留所有权利。
Actinobacillus pleuropneumoniae is the causative agent of acute and chronic pleuroneumonia that is responsible for substantial morbidity and mortality in the pig industry. New improved vaccines that can protect against all serotypes and prevent colonization are required. In a previous study we showed that whole cells of Propionibacterium acnes protected pigs from A. pleuropneumoniae serotype 1 and 5 and, therefore, the basis for a promising heterologous vaccine. The aim of this study was to identify those protein antigens of P. acnes responsible for protection against A. pleuropneumoniae infection. Six P. acnes protein antigens that were recognized by sera raised against A. pleuropneumoniae were identified by 2-DE and immunoblotting. Recombinant versions of all P. acnes proteins gave partial protection (10-80%) againstA. pleuropneumoniae serotype 1 and/or 5 infection in a mouse challenge model. The best protection (80% serotype 1; 60% serotype 5) was obtained using recombinant P. acnes single-stranded DNA-binding protein. In part, protection against A. pleuropneumoniae infection may be mediated by small peptide sequences present in P. acnes single-stranded DNA-binding protein that are cross-reactive with those present in the A. pleuropneumoniae-specific RTX toxin ApxIV and the zinc-binding protein ZnuA. The results suggest that P. acnes may be a useful vaccine to protect against different serotypes of A. pleuropneumoniae. (C) 2013 Elsevier Ltd. All rights reserved.