Analysis of a normalised expressed sequence tag (EST) library from a key pollinator, the bumblebee Bombus terrestris
Analysis of a normalised expressed sequence tag (EST) library from a key pollinator, the bumblebee Bombus terrestris
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DOI：
10.1186/1471-2164-11-110
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发表时间：
2010-02-15
期刊：
影响因子：
4.4
通讯作者：
Schmid-Hempel, Paul
中科院分区：
文献类型：
--
作者：
Sadd, Ben M.;Kube, Michael;Schmid-Hempel, Paul
背景：大黄蜂，Bombus terrestris（膜翅目），具有广泛的重要性。该物种在欧洲广泛用于商业授粉，与其他Bombus属植物一起是自然传粉昆虫组合的关键成员。此外，该物种在各种生物领域得到广泛研究。这个项目的目的是创建一个B. terrestris EST资源，将被证明是有价值的，以获得对这种重要的社会昆虫的更深入的了解。结果：构建了陆家鸡胸腹区标准化cDNA文库，加强了对稀有基因的发现。共测序了29428条ESTs。随后的聚类得到13’333个独特序列。其中，58.8%的蛋白质与已知蛋白质有显著的相似之处，54.5%的蛋白质与现有的膜翅目蛋白质序列“最吻合”。通过与蜜蜂和其他昆虫的比较，可以确定基因丢失、假基因进化和蜜蜂基因组中可能存在的不完整注释的潜在候选基因。此外，考虑到许多基础研究的重点以及对自然和商业人口的疾病威胁，大黄蜂的免疫系统是一个特别相关的组成部分。虽然该文库来自未受挑战的蜜蜂，但我们仍然发现了许多免疫基因的转录，这些基因跨越了主要描述的昆虫免疫途径。此外，EST文库为发现可用于种群水平研究的遗传标记提供了资源。事实上，最初的筛选确定了589个简单序列重复和854个潜在的单核苷酸多态性。结论：这些est所代表的资源对今后的工作有一定的参考价值。ESTs提供了转录活性区域的直接证据，但它们也将促进进一步的功能基因组学，基因发现和未来的基因组注释。这些都是更好地了解这一关键传粉媒介物种的重要方面。
Background: The bumblebee, Bombus terrestris (Order Hymenoptera), is of widespread importance. This species is extensively used for commercial pollination in Europe, and along with other Bombus spp. is a key member of natural pollinator assemblages. Furthermore, the species is studied in a wide variety of biological fields. The objective of this project was to create a B. terrestris EST resource that will prove to be valuable in obtaining a deeper understanding of this significant social insect.Results: A normalised cDNA library was constructed from the thorax and abdomen of B. terrestris workers in order to enhance the discovery of rare genes. A total of 29'428 ESTs were sequenced. Subsequent clustering resulted in 13'333 unique sequences. Of these, 58.8 percent had significant similarities to known proteins, with 54.5 percent having a "best-hit" to existing Hymenoptera sequences. Comparisons with the honeybee and other insects allowed the identification of potential candidates for gene loss, pseudogene evolution, and possible incomplete annotation in the honeybee genome. Further, given the focus of much basic research and the perceived threat of disease to natural and commercial populations, the immune system of bumblebees is a particularly relevant component. Although the library is derived from unchallenged bees, we still uncover transcription of a number of immune genes spanning the principally described insect immune pathways. Additionally, the EST library provides a resource for the discovery of genetic markers that can be used in population level studies. Indeed, initial screens identified 589 simple sequence repeats and 854 potential single nucleotide polymorphisms.Conclusion: The resource that these B. terrestris ESTs represent is valuable for ongoing work. The ESTs provide direct evidence of transcriptionally active regions, but they will also facilitate further functional genomics, gene discovery and future genome annotation. These are important aspects in obtaining a greater understanding of this key pollinator species.