SmPKC1, a new protein kinase C identified in the platyhelminth parasite Schistosoma mansoni
SmPKC1, a new protein kinase C identified in the platyhelminth parasite Schistosoma mansoni
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DOI：
10.1016/j.bbrc.2006.05.025
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发表时间：
2006-07-07
影响因子：
3.1
通讯作者：
Oliveira, Guilherme
中科院分区：
文献类型：
--
作者：
Bahia, Diana;Avelar, Livia;Oliveira, Guilherme
曼氏血吸虫的信号转导通路是开发新的控制策略对抗这种人类扁形蠕虫寄生虫的靶分子的有前途的来源。蛋白激酶C（PKC）家族成员在受体酪氨酸激酶和其他受体激活的这些途径中发挥关键作用，控制各种生理过程。在这里，我们报告的克隆和分子特征的第一个PKC确定在S。mansoni结构分析表明，SmPKC 1具有传统PKC亚家族的所有典型特征。基因结构在计算机上确定，并发现包括总共15个外显子和14个内含子。这种结构是高度保守的;所有内含子位置也存在于人PKC β基因中，并且大多数外显子大小是相同的。使用基因组DNA上的PCR，我们能够表明，假定的直向同源的SmPKC 1存在于9个血吸虫物种。SmPKC 1的表达是发育调节与毛蚴中的最高水平的成绩单，而SmPKC 1蛋白的表达是较高的孢子囊。SmPKC 1的定位孢子囊脊细胞和在adjacomula髋臼腺表明，该酶起着与幼虫转化相关的信号转导途径中的作用。(c)2006年爱思唯尔公司All rights reserved.
Schistosoma mansoni signal transduction pathways are promising sources of target molecules for the development of novel control strategies against this platyhelminth parasite of humans. Members of the protein kinase C (PKC) family play key roles in such pathways activated by both receptor tyrosine kinases and other receptors, controlling a variety of physiological processes. Here, we report the cloning and molecular characterization of the first PKC identified in S. mansoni. Structural analysis indicated that SmPKC1 exhibits all the features typical of the conventional PKC subfamily. The gene structure was determined in silico and found to comprise a total of 15 exons and 14 introns. This structure is highly conserved; all intron positions are also present in the human PKC beta gene and most of the exon sizes are identical. Using PCR on genomic DNA we were able to show that putative orthologues of SmPKC1 are present in 9 Schistosoma species. SmPKC1 expression is developmentally regulated with the highest level of transcripts in miracidia, whereas SmPKC1 protein expression is higher in the sporocyst. The localization of SmPKC1 on the sporocyst ridge cyton and in schistosomula acetabular glands suggests that the enzyme plays a role in signal transduction pathways associated with larval transformation. (c) 2006 Elsevier Inc. All rights reserved.