Fluorescent-magnetic poly(poly(ethyleneglycol)monomethacrylate)-grafted Fe3O4 nanoparticles from post-atom-transfer-radical-polymerization modification: synthesis, characterization, cellular uptake and imaging
Fluorescent-magnetic poly(poly(ethyleneglycol)monomethacrylate)-grafted Fe3O4 nanoparticles from post-atom-transfer-radical-polymerization modification: synthesis, characterization, cellular uptake and imaging
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DOI：
10.1039/c2jm15768c
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发表时间：
2012-03
影响因子：
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通讯作者：
Xiaomei Lu;Rongcui Jiang;Quli Fan;Lei Zhang;Hongming Zhang;Minhua Yang;Yanwen Ma;Lianhui Wang;Wei Huang
中科院分区：
文献类型：
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作者：
Xiaomei Lu;Rongcui Jiang;Quli Fan;Lei Zhang;Hongming Zhang;Minhua Yang;Yanwen Ma;Lianhui Wang;Wei Huang
采用无溶剂原子转移自由基聚合（ATRP）法合成了水溶性聚乙二醇单甲基丙烯酸酯（P（PEGMA）-grafted）Fe 3 O 4纳米粒子，并以3-氨丙基三甲氧基硅烷为锚分子对其进行了表面修饰，使其具有一定的NH 2基团。然后通过将荧光素异硫氰酸酯（FITC）共价键合到NH 2基团上获得荧光磁性纳米颗粒（MNP）。从FT-IR和XPS分析中确定了MNP表面的成功改性，表明这种用于引入NH 2基团的简便的后ATRP改性方法将扩展通过ATRP方法生产的聚合物包覆的MNP的潜在应用。所合成的FITC接枝的MNP（FITC-MNP）具有良好的水溶性和稳定性，并且具有均匀的流体动力学粒径（36.2 ± 2.2 nm）。这些纳米颗粒是超顺磁性的，饱和磁化强度（Ms）为23 emu g−1，足以用于生物应用。巨噬细胞对荧光MNP的摄取约为2 pg Fe/细胞，这与具有良好生物相容性的原始P（PEGMA）接枝的MNP几乎相似。此外，使用3 T3成纤维细胞的MMT测定表明FITC-MNP的低细胞毒性作用。FITC-MNP可以被乳腺癌细胞有效地摄取高达85 pg Fe/细胞，这可能是由于P（PEGMA）链在细胞膜中的高溶解度。共聚焦显微镜结果显示，FITC-MNP位于乳腺癌细胞内，但不在细胞膜内。这些结果表明，具有荧光和磁性功能的FITC-MNP在生物成像中具有巨大的应用潜力。
Water-soluble poly(poly(ethyleneglycol)monomethacrylate)-grafted (P(PEGMA)-grafted) Fe3O4 nanoparticles synthesized via a solvent-free atom transfer radical polymerization (ATRP) method were conveniently surface-modified with 3-aminopropyltrimethoxysilane as anchor molecules to donate NH2 groups. Fluorescent magnetic nanoparticles (MNPs) were then obtained by covalently bonding fluorescein isothiocyanate (FITC) to the NH2 groups. The successful modification of the MNP surface was ascertained from FT-IR and XPS analyses, indicating that such a facile post-ATRP modification approach for introducing NH2 groups will extend the potential applications of polymer-coated MNPs produced via the ATRP method. The as-synthesized FITC-grafted MNPs (FITC-MNPs) showed good water solubility and stability, and have a uniform hydrodynamic particle size of 36.2 ± 2.2 nm. These nanoparticles are superparamagnetic with a saturation magnetization (Ms) of 23 emu g−1, which is sufficient for bioapplications. The uptake of the fluorescent MNPs by macrophage cells is about 2 pg Fe/cell, which is nearly similar to the pristine P(PEGMA)-grafted MNPs with good biocompatibility. Furthermore, an MMT assay using the 3T3 fibroblasts indicates the low cytotoxic effect of the FITC-MNPs. The FITC-MNPs can be efficiently uptaken by breast cancer cells up to 85 pg Fe/cell, which might be due to the high solubility of the P(PEGMA) chains in the cell membranes. Confocal microscope results showed that the FITC-MNPs were located inside the breast cancer cells but not within the cell membranes. These results indicate that FITC-MNPs with both fluorescence and magnetic functionalities have great potential for applications in bioimaging.