The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth
The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth
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DOI：
10.1016/j.watres.2008.02.021
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发表时间：
2008-06-01
期刊：
影响因子：
12.8
通讯作者：
Hu, Zhiqiang
中科院分区：
文献类型：
--
作者：
Choi, Okkyoung;Deng, Kathy Kanjun;Hu, Zhiqiang
新兴的纳米材料受到污水处理设施和环境的极大关注。利用现有的呼吸测量法和自动微量荧光测定法评价了银纳米颗粒和其他重要的银物种对微生物生长的抑制作用。使用控制良好的连续运行的生物反应器中的自养硝化菌，银纳米粒(平均尺寸为14+/-6 nm)、银离子(AgNO3)和氯化银胶体(平均尺寸=0.25um m)均为1 mg/L，对呼吸作用的抑制程度分别为86+/-3%、42+/-7%和46+/-4%。经长期微量滴度测定，在约0.5 mg/L的浓度下，纳米银、银离子和氯化银胶体对大肠杆菌PHL628-GFP的生长抑制率分别为55+/-8%、100%和66+/-6%。通过使用活/死的BacLight(TM)细菌活力测试，细胞膜的完整性在测试Ag物种的处理下不会受到损害。然而，电子显微镜显示，银纳米颗粒附着在微生物细胞上，可能导致细胞壁点蚀。结果表明，硝化细菌特别容易受到银纳米颗粒的抑制，银纳米颗粒的积累可能会对废水处理中的微生物产生不利影响。(C)2008爱思唯尔有限公司。保留所有权利。
Emerging nanomaterials are of great concern to wastewater treatment utilities and the environment. The inhibitory effects of silver nanoparticles (Ag NPs) and other important Ag species on microbial growth were evaluated using extant respirometry and an automatic microtiter fluorescence assay. Using autotrophic nitrifying organisms from a well-controlled continuously operated bioreactor, Ag NPs (average size = 14 +/- 6 nm), Ag+ ions (AgNO3), and AgCl colloids (average size = 0.25 mu m), all at 1 mg/L Ag, inhibited respiration by 86 +/- 3%, 42 +/- 7%, and 46 +/- 4%, respectively. Based on a prolonged microtiter assay, at about 0.5 mg/L Ag, the inhibitions on the growth of Escherichia coli PHL628-gfp by Ag NPs, Ag ions, and Agcl colloids were 55 +/- 8%, 100%, and 66 +/- 6%, respectively. Cell membrane integrity was not compromised under the treatment of test Ag species by using a LIVE/DEAD Baclight(TM) bacterial viability assay. However, electron micrographs demonstrated that Ag NPs attached to the microbial cells, probably causing cell wall pitting. The results suggest that nitrifying bacteria are especially susceptible to inhibition by Ag NPs, and the accumulation of Ag NPs could have detrimental effects on the microorganisms in wastewater treatment. (C) 2008 Elsevier Ltd. All rights reserved.