Integration host factor binds specifically to sites in the ilvGMEDA operon in Escherichia coli.
Integration host factor binds specifically to sites in the ilvGMEDA operon in Escherichia coli.
复制标题
整合宿主因子与大肠杆菌 ilvGMEDA 操纵子中的位点特异性结合。
DOI：
10.1016/0022-2836(88)90212-4
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发表时间：
1988
影响因子：
5.6
通讯作者：
Freundlich,M
中科院分区：
文献类型：
--
作者：
Tsui,P;Freundlich,M
大肠杆菌整合宿主因子（IHF）是一种组蛋白样蛋白，参与位点特异性重组和调节许多噬菌体和细菌基因的表达。我们以前已经表明，在E. IHF突变体中大肠杆菌的数量大大减少。我们在这里报告说，IHF特异性保护ilvGMEDA启动子调节区域内的两个位点免受DNA酶I消化。这些位点位于启动子的上游，并位于前导区中，正好在指定衰减子的序列之前。足迹实验和凝胶阻滞实验表明，这些位点对IHF有很强的亲和力。ilvGMEDA-Iac启动子融合的这些数据和结果表明IHF在ilvGMEDA-Iac操纵子表达中的直接作用。
Integration host factor (IHF) ofEscherichia coliis a histone-like protein that is involved both in site-specific recombination and in regulating the expression of a number of phage and bacterial genes. We have shown previously that transcription of theilvGMEDAoperon inE. coliis greatly reduced in IHF mutants. We report here that IHF specifically protects two sites within theilvGMEDApromoter-regulatory region against DNase I digestion. These sites are located upstream from the promoter and in the leader region just prior to the sequence that specifies the attenuator. The footprinting experiments and gel retardation assays show that these sites have strong affinity for IHF. These data and results withilvGMEDA-lacpromoter fusions suggest a direct role for IHF in expression of theilvGMEDAoperon.