Peripheral T cell lymphomas with follicular T helper phenotype: a new basket or a distinct entity? Revising Karl Lennert's personal archive
Peripheral T cell lymphomas with follicular T helper phenotype: a new basket or a distinct entity? Revising Karl Lennert's personal archive
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DOI：
10.1111/j.1365-2559.2011.03981.x
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发表时间：
2011-10-01
期刊：
影响因子：
6.4
通讯作者：
Pileri, Stefano A.
中科院分区：
文献类型：
--
作者：
Agostinelli, Claudio;Hartmann, Sylvia;Pileri, Stefano A.
目的：对从卡尔·伦纳特个人档案(21例)和博洛尼亚和法兰克福登记处(4例)中选择的25例细胞学相似的病例进行修订。方法和结果：所有病例均经石蜡包埋，并进行免疫组织化学和分子技术研究。虽然表型分析信息量很大，但在分子研究中，只有EBER原位杂交(ISH)成功。22例为外周T细胞淋巴瘤(PTCL)。其中6例为血管免疫母细胞T细胞淋巴瘤(AITL)，13例为PTCL，未明确分类(NOS)，包括4例滤泡型和1例T带型肿瘤，3例为介于AITL和PTCL/NOS之间的交界性肿瘤。所有病例均为均一的中小型细胞，核异型性较轻，胞质边缘明显透明/苍白。在免疫组织化学中，他们定期表达三到六个滤泡辅助T细胞(FTH)相关标记。EBER-ISH显示，除滤泡型生长模式外，所有肿瘤中都有分散的EBV感染的B细胞。滤泡树突状细胞和高内皮微静脉的含量因组织类型的不同而有显著差异。结论：本研究表明：(I)历史资料仍可用于临床，(Ii)FTH表型对应于广泛的PTCL，可能形成一个新的类别，有待在未来的分子和临床病理分析中验证。
Aims: To revise 25 cases selected from Karl Lennert's personal archive (21) and Bologna and Frankfurt Registries (four) because of cytological similarities.Methods and results: All cases were provided with paraffin blocks and studied by immunohistochemistry and molecular techniques. While phenotyping was very informative, among molecular studies only EBER in situ-hybridization (ISH) was successful. Twenty-two cases were concluded as peripheral T cell lymphomas (PTCL). Of these, six were reclassified as angioimmunoblastic T cell lymphoma (AITL), 13 as PTCL, not otherwise specified (NOS), including four follicular variants and one tumour with T-zone pattern, and three as borderline tumours between AITL and PTCL/NOS. All these cases consisted homogeneously of small/medium-sized elements with mild nuclear atypia and an evident rim of clear/pale cytoplasm. On immunohistochemistry, they regularly expressed three to six follicular helper T cell (FTH)-associated markers. EBER-ISH revealed scattered EBV-infected B cells in all tumours except those with 'follicular' growth pattern. The content of follicular dendritic cells and high-endothelial venules varied significantly depending on the histotype.Conclusions: This study shows that: (i) historical material can be still employed usefully, and (ii) the FTH-phenotype corresponds to a broad spectrum of PTCLs that might form a new category to be validated in future molecular and clinicopathological analyses.