The in vivo effects of bisGMA on murine uterine weight, nucleic acids and collagen.
The in vivo effects of bisGMA on murine uterine weight, nucleic acids and collagen.
复制标题
bisGMA 对小鼠子宫重量、核酸和胶原蛋白的体内影响。
DOI：
10.1046/j.0909-8836.1998.eos106607.x
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Johnson,S
中科院分区：
文献类型：
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作者：
Mariotti,A;Soderholm,KJ;Johnson,S
本研究的目的是评估商业bisGMA刺激雌激素敏感性靶组织生长的能力。切除成年雌性Swiss-Webster小鼠的卵巢，并接受油、雌二醇（100 μ g/kg）或两种bisGMA剂量之一（25 μ g/kg或100 μ g/kg）。从手术当天开始，每周3次皮下注射激素、药物或油。处理3周后，处死动物，取出子宫，称重并储存在-80 ℃下用于生化分析。接受高剂量（100 μ g/kg）bisGMA或雌二醇的卵巢切除小鼠的子宫显示标准化湿重显著增加，分别比卵巢切除对照子宫标准化重量增加29%和786%。在低bisGMA剂量（25 μ g/kg）组中，标准化子宫湿重与卵巢切除对照相比无统计学显著性。子宫组织的生化分析表明，雌二醇导致维持DNA含量，RNA含量，RNA/DNA比和胶原蛋白含量显着高于卵巢切除对照组。无论是低剂量还是高剂量的bisGMA刺激的RNA含量、DNA含量或RNA/DNA比率都不高于卵巢切除对照。然而，高剂量（100 μ g/kg）的bisGMA导致子宫胶原蛋白含量显著增加，高于卵巢切除对照组。
The aim of this investigation was to evaluate the ability of commercial bisGMA to stimulate growth in an estrogen-sensitive target tissue. Adult, female, Swiss-Webster mice were ovariectomized and received either oil, estradiol (100 microg/kg), or one of two bisGMA doses (25 microg/kg or 100 microg/kg). Starting on the day of surgery, the hormone, drug or oil was injected subcutaneously 3 times a week. After 3 wk of treatment, the animals were sacrificed, the uteri removed, weighed and stored at-80 degrees C for biochemical analysis. The uteri from ovariectomized mice receiving high dose (100 microg/kg) bisGMA or estradiol showed a significant increase in normalized wet weight that was 29% and 786%, respectively, greater than the ovariectomized control uterine normalized weights. In the low bisGMA dose (25 microg/kg) group, normalized uterine wet weights were not statistically significant from ovariectomized controls. Biochemical analyses of uterine tissues revealed that estradiol resulted in maintaining DNA content, RNA content, RNA/DNA ratios and collagen content significantly above the ovariectomized control. Neither the low nor high doses of bisGMA stimulated RNA content, DNA content or RNA/DNA ratios above ovariectomized controls. However, the high dose (100 microg/kg) of bisGMA caused a significant increase above ovariectomized controls in uterine collagen content.
影响因子：
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作者：
K. Krempen;Doris Grotkopp;Keith Hall;Alexandra J. Bache;Andrea Gillan;R. Rippe;D. Brenner;M. Breindl
通讯作者：
K. Krempen;Doris Grotkopp;Keith Hall;Alexandra J. Bache;Andrea Gillan;R. Rippe;D. Brenner;M. Breindl