Multivariate optimization of a spectrophotometric method for copper determination in Brazilian sugar-cane spirits using the Doehlert design
Multivariate optimization of a spectrophotometric method for copper determination in Brazilian sugar-cane spirits using the Doehlert design
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DOI：
10.1016/j.microc.2011.04.008
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发表时间：
2011-09
影响因子：
4.8
通讯作者：
L. F. S. Caldas;B. Francisco;A. Netto;R. Cassella
中科院分区：
文献类型：
--
作者：
L. F. S. Caldas;B. Francisco;A. Netto;R. Cassella
本工作报告了一个多元优化的分光光度法测定甘蔗酒中的铜，探讨在十六烷基氯化吡啶（CPC）和Triton X-100的存在下，铜（II）与苯基荧光酮（PF）的反应。的优化策略是基于应用程序的两个水平的全因子设计的显着变量的筛选，然后由一个Doehlert设计，以确定最佳条件。当pH为8.0、PF和TX-100浓度分别为6.2 × 10 - 5和1.0 × 10 - 2 molL-1时，灵敏度最高。方法的检出限为3.4 μg L-1，定量限为11 μg L-1，用于17个样品中铜的测定。将所得结果与ICP OES所得结果进行比较，未观察到统计学差异。
This present work reports a multivariate optimization of a spectrophotometric method for copper determination in sugar-cane spirits, exploring the reaction of Cu(II) with phenylfluorone (PF) in the presence of cetylpyridinium chloride (CPC) and Triton X-100. The optimization strategy was based on the application of a two-level full factorial design for the screening of significant variables followed by a Doehlert design to determine the optimum conditions. The highest sensitivity was achieved when the pH was 8.0 and the PF and TX-100 concentrations were 6.2 × 10− 5and 1.0 × 10− 2mol L− 1, respectively. The optimized method presented a limit of detection of 3.4 μg L− 1and a limit of quantification of 11 μg L− 1and was applied in the determination of Cu in 17 samples. The obtained results were compared with those obtained by ICP OES and no statistical difference between the methods was observed.