Genomewide investigation into DNA elements and ABC transporters involved in imazalil resistance in Penicillium digitatum
Genomewide investigation into DNA elements and ABC transporters involved in imazalil resistance in Penicillium digitatum
复制标题
全基因组研究涉及指状青霉抑霉唑抗性的 DNA 元件和 ABC 转运蛋白
DOI：
10.1111/1574-6968.12235
复制
发表时间：
2013-11-01
影响因子：
2.1
通讯作者：
Yu, Dongliang
中科院分区：
文献类型：
--
作者：
Sun, Xuepeng;Ruan, Ruoxin;Yu, Dongliang
指状青霉（Penicilliumdigitatum）是柑橘绿色霉菌的主要病原菌之一。由于抗杀真菌剂菌株的扩散，疾病控制变得不那么有效。然而，其抗性机制的基因组规模的分析是稀缺的。本研究对R1基因型菌株Pd 01-ZJU进行了全基因组测序，并对与耐药性高度相关的基因和DNA元件进行了研究。结果表明，指状假单胞菌耐药相关DNA元件在拷贝数和染色体位置上均存在变异，表明指状假单胞菌最近频繁的易位可能与环境适应有关。此外，还对Pd 01-ZJU中的ABC转运蛋白进行了鉴定，并利用实时荧光定量PCR分析了典型亚家族（ABCG、ABCC和ABCB）在抑霉唑抗性中的作用。七ABC蛋白，包括先前表征的PMR 1和PMR 5，诱导抑霉唑，这表明在耐药性中的作用。综上所述，本研究首次系统地揭示了R1基因型指状青霉的基因组信息，并对抑霉唑抗性相关的DNA元件和ABC蛋白进行了研究，为研究其他病原真菌的抑霉唑抗性机制提供了参考。
Penicillium digitatum, causing citrus green mold, is one of the most devastating pathogenic fungi for postharvest fruits. The disease control is becoming less efficient because of the dispersal of fungicide-resistant strains. However, genome-scale analyses of its resistance mechanism are scarce. In this work, we sequenced the whole genome of the R1 genotype strain Pd01-ZJU and investigated the genes and DNA elements highly associated with drug resistance. Variation in DNA elements related to drug resistance between P.digitatum strains was revealed in both copy number and chromosomal location, indicating that their recent and frequent translocation might have contributed to environmental adaptation. In addition, ABC transporter proteins in Pd01-ZJU were characterized, and the roles of typical subfamilies (ABCG, ABCC, and ABCB) in imazalil resistance were explored using real-time PCR. Seven ABC proteins, including the previously characterized PMR1 and PMR5, were induced by imazalil, which suggests a role in drug resistance. In summary, this work presents genome information of the R1 genotype P.digitatum and systematically investigates DNA elements and ABC proteins associated with imazalil resistance for the first time, which would be indicative for studying resistant mechanisms in other pathogenic fungi.