The use of two novel methods to grow protein crystals by microdialysis and vapor diffusion in an agarose gel.
The use of two novel methods to grow protein crystals by microdialysis and vapor diffusion in an agarose gel.
复制标题
使用两种新方法通过微透析和琼脂糖凝胶中的蒸汽扩散来生长蛋白质晶体。
DOI：
10.1107/s0907444994001332
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Thiessen Kj
中科院分区：
文献类型：
--
作者：
Thiessen Kj
: The crystals of most proteins are poorly ordered and diffract to lower resolutions than other crystals of simple and inorganic compounds. The use of two novel methods for gel protein crystal growth, utilizing liquid diffusion and vapor diffusion, are described for the growth of lysozyme and canavalin. Crystallization using gels has been demonstrated to improve crystal quality by reducing convective flow, sedimentation, nucleation and twinning. Preliminary X-ray diffraction data are also presented.
