Bioengineered vascular graft grown in the mouse peritoneal cavity.
Bioengineered vascular graft grown in the mouse peritoneal cavity.
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DOI：
10.1016/j.jvs.2010.05.015
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发表时间：
2010-10
影响因子：
4.3
通讯作者：
Sharifi, Behrooz G.
中科院分区：
文献类型：
--
作者：
Song, Lei;Wang, Lai;Shah, Prediman K.;Chaux, Aurelio;Sharifi, Behrooz G.
We tested the hypothesis that the mouse peritoneum can function like a bioreactor to generate directed bio-engineered tissues such as those used for bypass grafting. Additionally, we reasoned that the mouse animal model would allow us to elucidate the underlying cellular and molecular mechanisms that are responsible for the generation of tissue in peritoneal cavity. Plastic tubes (2 tubes/mouse) were implanted into the peritoneal cavity of 3 strains of mice (C57BL/6, BALB/c, and MRL). The tubes were harvested, tissue capsule surrounding the tubes was removed, and analyzed by immunostaining (5 capsules/5 mice/strain) and microarray (3 capsules/3 mice/strain). In addition, the tissue capsules that were harvested from MRL mice (n=21) were grafted into abdominal aorta of the same mice as autografts. The patency of all grafts was monitored by micro-ultrasound and their functionality was assessed by Laser Doppler Imaging of blood flow in femoral arteries. Venous (n=13) and arterial isografts (n=11) were used as positive controls. In a negative control group (5 mice/strain), the abdominal aorta was occluded by double ligation with 9-0 silk. The implanted plastic tubes required at least 8 weeks of incubation in the peritoneum of the 3 strains of mice in order to generate useful grafts. No vascular cells. were found in the tissue capsules. Microarray analysis of tissue capsules revealed that the capsular cells express a gene expression program that is vastly shared among the 3 strains of mice and the cells exhibit high degree of plasticity. The micro-ultrasound analysis of the grafts showed that 62% of autografts remained patent compared to 77% of venous isografts and 91% of arterial isografts. The Laser Doppler Imaging analysis showed that blood flow dropped by 40% and 35% in the autografts and vein isografts, respectively, one day after surgery. The flow, however, rebounded to the level of arterial isografts one month post surgery and remained unchanged among all grafts for the next 4 months. Immunostaining of the autografts showed a thick vessel wall with endothelial cells that lined the lumen and smooth muscle cells that constituted the graft wall. The mouse peritoneal cavity of mice has the ability to function like a bioreactor to generate bio-engineered tissues. The tissue capsules harvested from peritoneal cavity of a mouse are composed of nonvascular cells that display phenotype of progenitor cells. After grafting, however, the capsule auto-grafts become arterialized and remained patent for at least 4 months after surgery, similar to venous or arterial iso-grafts.
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