PARA-NONYL-PHENOL - AN ESTROGENIC XENOBIOTIC RELEASED FROM MODIFIED POLYSTYRENE
PARA-NONYL-PHENOL - AN ESTROGENIC XENOBIOTIC RELEASED FROM MODIFIED POLYSTYRENE
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DOI：
10.2307/3431154
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发表时间：
1991-05-01
影响因子：
10.4
通讯作者：
SONNENSCHEIN, C
中科院分区：
文献类型：
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作者：
SOTO, AM;JUSTICIA, H;SONNENSCHEIN, C
烷基酚被广泛用作塑料助剂和表面活性剂。我们报告鉴定烷基酚，壬基酚，作为一种雌激素物质从塑料离心管释放。该化合物经甲醇提取，闪蒸色谱和反相高效液相色谱纯化，气相色谱-质谱联用鉴定。壬基酚诱导人雌激素敏感MCF7乳腺肿瘤细胞增殖和孕酮受体。壬基酚还能触发大鼠子宫内膜的有丝分裂活性；这一结果证实了MCF7细胞增殖生物测定的可靠性。烷基酚，特别是壬基酚的雌激素特性表明，在实验和诊断测试中使用含有这些化学品的塑料器皿可能会产生虚假的结果，而且这些化合物以及烷基酚聚氧基酸盐也可能对接触的人类和整个环境造成潜在危害。
Alkylphenols are widely used as plastic additives and surfactants. We report the identification of an alkylphenol, nonylphenol, as an estrogenic substance released from plastic centrifuge tubes. This compound was extracted with methanol, purified by flash chromatography and reverse-phase high performance liquid chromatography, and identified by gas chromatography-mass spectrometry. Nonylphenol induced both cell proliferation and progesterone receptor in human estrogen-sensitive MCF7 breast tumor cells. Nonylphenol also triggered mitotic activity in rat endometrium; this result confirms the reliability of the MCF7 cell proliferation bioassay. The estrogenic properties of alkylphenols, specifically nonylphenols, indicate that the use of plasticware containing these chemicals in experimental and diagnostic tests may lead to spurious results, and these compounds as well as alkylphenol polyethoxylates may also be potentially harmful to exposed humans and the environment at large.