Advances in CRISPR-based SERS detection of food contaminants: A review
Advances in CRISPR-based SERS detection of food contaminants: A review
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DOI：
10.1016/j.tifs.2023.07.001
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发表时间：
2023-07
期刊：
影响因子：
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通讯作者：
Mehdi Hajikhani;Yi Zhang;Xuefei Gao;Mengshi Lin
中科院分区：
文献类型：
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作者：
Mehdi Hajikhani;Yi Zhang;Xuefei Gao;Mengshi Lin
CRISPR-Cas系统和表面增强拉曼光谱（Sers）的集成已经成为开发高灵敏度生物传感器的有力方法。本文综述了CRISPR-Cas 12 a和Cas 13 a系统与Sers技术相结合在检测核酸方面的潜力。范围和方法本文综述了CRISPR-Cas 12 a和Cas 13 a系统在医疗保健、食品安全、环境监测和生物防御等各个领域的应用研究。综述的研究强调了CRISPR-Cas系统靶向特定DNA或RNA序列的能力，而Sers则是一种高度灵敏的检测方法。这些技术的结合使生物传感器的发展，用于检测病原体，遗传标记，耐药基因，化学化合物，和食品中的掺假物。关键发现和conclusionsThe审查的研究表明，重大发现和潜力的整合CRISPR-Cas和Sers技术的生物传感应用。这些研究展示了CRISPR-Cas 12 a和Cas 13 a系统以高灵敏度和特异性检测核酸的能力，即使在痕量水平。使用Sers作为检测方法提供了诸如增强的灵敏度、选择性和可靠性的优点。CRISPR-Cas和Sers技术的结合有可能彻底改变各个领域的诊断测试。尽管挑战依然存在，包括提高检测限和减少复杂基质的干扰，但这些生物传感器在早期疾病诊断、病原体检测和环境监测方面的前景是显而易见的。进一步的研究和开发是必要的，以充分利用CRISPR-Cas和Sers技术的潜力，用于未来的生物传感应用。
BackgroundThe integration of CRISPR-Cas systems and surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful approach for developing highly sensitive biosensors. This review explores recent studies that demonstrate the potential of CRISPR-Cas12a and Cas13a systems combined with SERS technology in detecting nucleic acids with exceptional sensitivity and specificity.Scope and approachThis review examines studies that showcase the application of CRISPR-Cas and SERS technology in various fields, including healthcare, food safety, environmental monitoring, and biodefense. The reviewed studies highlight the capability of CRISPR-Cas systems to target specific DNA or RNA sequences, while SERS serves as a highly sensitive detection method. The combination of these technologies enables the development of biosensors for the detection of pathogens, genetic markers, drug resistance genes, chemical compounds, and adulterants in food.Key findings and conclusionsThe reviewed studies demonstrate the significant findings and potential of integrating CRISPR-Cas and SERS technologies for biosensing applications. The studies showcase the ability of CRISPR-Cas12a and Cas13a systems to detect nucleic acids with high sensitivity and specificity, even at trace levels. The use of SERS as a detection method offers advantages such as enhanced sensitivity, selectivity, and reliability. The combination of CRISPR-Cas and SERS technology has the potential to revolutionize diagnostic testing in various sectors. Although challenges remain, including improving detection limits and reducing interference from complex matrices, the promise of these biosensors in early disease diagnosis, pathogen detection, and environmental monitoring is evident. Further research and development are warranted to harness the full potential of CRISPR-Cas and SERS technology for future biosensing applications.