Salinicola zeshunii sp. nov., a Moderately Halophilic Bacterium Isolated from Soil of a Chicken Farm
Salinicola zeshunii sp. nov., a Moderately Halophilic Bacterium Isolated from Soil of a Chicken Farm
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DOI：
10.1007/s00284-012-0259-4
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发表时间：
2013-02
影响因子：
2.6
通讯作者：
Li Cao;Qiuxiang Yan;Hai-yan Ni;Gang Hu;Q. Hong;Shun-Peng Li
中科院分区：
文献类型：
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作者：
Li Cao;Qiuxiang Yan;Hai-yan Ni;Gang Hu;Q. Hong;Shun-Peng Li
对从中国某养鸡场土壤中分离到的一株中度嗜盐菌N4 T进行了分类鉴定。革兰氏阴性，无孢子形成，能动，杆状。基于16 S rRNA基因序列的系统发育分析表明，该菌株与Salinicola salariesM 27 T（98.3%）、Salinicola sociusSMB 35 T（98.1%）和Salinicola halophilusCG4.1T（98.1%）的同源性最高，属于Salinicola属。N4 T菌株的DNA G+C含量为69.1mol%，其细胞脂肪酸主要为C16：0（25.6%）、C18：1ω 7 c（35.0%）和C19：0环ω 8 c（11.9%），这是盐生菌属成员共有的特征。N4 T菌株与Salinicola salariesM 27 T、Salinicola sociusSMB 35 T和Salinicola halophilusCG4.1T的DNA-DNA同源性分别为34.3%、28.7%和26.9%。根据表型、化学分类学、DNA-DNA亲缘关系和系统发育分析结果，N4 T菌株应被分类为Salinicola属的一个新种，命名为Salinicola zeshuniisp。nov.，模式菌株为N4 T（=KACC 16602 T = CCTCC AB 2012912 T）。
The taxonomic status of a moderately halophilic bacterium, strain N4T, isolated from soil of a chicken farm in China was determined. It was Gram-negative, non-spore-forming, motile, and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequence indicated that this strain belonged to the genusSalinicola, as it showed the highest sequence similarities toSalinicola salariesM27T(98.3 %),Salinicola sociusSMB35T(98.1 %), andSalinicola halophilusCG4.1T(98.1 %). The major cellular fatty acids were C16:0(25.6 %), C18:1ω7c (35.0 %), and C19:0cycloω8c(11.9 %), which are properties shared by members of the genusSalinicola.The DNA G+C content of strain N4Twas 69.1 mol %. The level of DNA–DNA relatedness between strain N4Tand the other three type strains of the genus ofSalinicola salariesM27T,Salinicola sociusSMB35T, andSalinicola halophilusCG4.1Twere 34.3, 28.7, and 26.9 %, respectively. Based on the results of phenotypic, chemotaxonomic, DNA–DNA relatedness, and phylogenetic analysis, strain N4Tshould be classified as a novel species of the genusSalinicola, for which the nameSalinicola zeshuniisp. nov. is proposed, with strain N4T(=KACC 16602T= CCTCC AB 2012912T) as the type strain.