The Staphylococcus aureus allelic genetic loci for serotype 5 and 8 capsule expression contain the type-specific genes flanked by common genes
The Staphylococcus aureus allelic genetic loci for serotype 5 and 8 capsule expression contain the type-specific genes flanked by common genes
复制标题
DOI：
10.1099/00221287-143-7-2395
复制
发表时间：
1997-07-01
期刊：
影响因子：
--
通讯作者：
Lee, CY
中科院分区：
文献类型：
--
作者：
Sau, S;Bhasin, N;Lee, CY
分别测定了金黄色葡萄球菌5型和8型荚膜多糖（CP）合成中涉及的两个基因簇（cap 5和cap 8）的核苷酸序列。每个基因簇包含16个开放阅读框，5型CP的开放阅读框命名为cap 5A至cap 5 P，8型CP的开放阅读框命名为cap 8A至cap 5 P。cap 5和cap 8基因座是等位的，并被定位到葡萄球菌的标准Smal图谱中的Smal-G片段。金黄色葡萄球菌菌株NCTC 8325。预测的cap 5A至cap 5G和cap 5L至cap 5 P的基因产物分别与cap 8A至cap 8 G和cap 8L至cap 8 P的那些基本上相同，具有非常少的氨基酸取代。位于每个位点中心区域的4个ORF具有类型特异性。将cap 5和cap 8的预测氨基酸序列与数据库中发现的序列进行比较，可以将功能暂定分配给16个ORF中的15个。大多数的胶囊基因可能参与氨基糖的合成;其余的可能参与糖转移，胶囊链长度调节，聚合和运输。
The nucleotide sequences of two gene clusters, cap5 and cap8, involved in the synthesis of Staphylococcus aureus type 5 and type 8 capsular polysaccharides (CPs), respectively, were determined. Each gene cluster contained 16 ORFs, which were named cap5A through cap5P for type 5 CP and cap8A through cap5P for type 8 CP. The cap5 and cap8 loci were allelic and were mapped to the Smal-G fragment in the standard Smal map of Staph. aureus strain NCTC 8325. The predicted gene products of cap5A through cap5G and cap5L through cap5P are essentially identical to those of cap8A through cap8G and cap8L through cap8P, respectively, with very few amino acid substitutions. Four ORFs located in the central region of each locus are type-specific. A comparison of the predicted amino acid sequences of cap5 and cap8 with sequences found in the databases allowed tentative assignment of functions to 15 of the 16 ORFs. The majority of the capsule genes are likely to be involved in amino sugar synthesis; the remainder are likely to be involved in sugar transfer, capsule chain-length regulation, polymerization and transport.