Expression of a candidate marker for progenitor cells, Musashi-1, in the proliferative regions of human antrum and its decreased expression in intestinal metaplasia
Expression of a candidate marker for progenitor cells, Musashi-1, in the proliferative regions of human antrum and its decreased expression in intestinal metaplasia
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DOI：
10.1111/j.1365-2559.2005.02223.x
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发表时间：
2005-10-01
期刊：
影响因子：
6.4
通讯作者：
Kitajima, M
中科院分区：
文献类型：
--
作者：
Akasaka, Y;Saikawa, Y;Kitajima, M
目的：人类胃造祖细胞的可靠标记物尚未阐明。本研究旨在阐明新近提出的小鼠肠道干细胞标记物Musashi-1(MSI-1)是否可作为人胃祖细胞的标记物。方法与结果：免疫组织化学结果显示，MSI-1+细胞主要在成人胃窦峡部/颈部(干细胞的可能位置)检测到，但仅限于发育早期胎儿幽门腺体的基底区。这些结果表明，MSI-1的表达特异性地发生在含有干细胞的区域。在成人胃窦峡部/颈区，MSI-1+细胞与增殖细胞核抗原(PCNA)+细胞混合，但不同时表达增殖细胞核抗原和Ki67。MSI-1的表达与MUC5AC和MUC6的表达部分重叠，表明MSI-1+细胞保留了凹陷和幽门腺细胞分化表型的某些特征。结论：MSI-1在增殖区的特异性表达提示MSI-1是人胃窦部活跃增殖前具有祖细胞特征的细胞标志物。
Aim: Reliable makers for progenitor cells in the human stomach have not been elucidated. The aim of the present study was to clarify whether Musashi-1 (Msi-1), which has recently been proposed as a stem cell marker in mouse intestine, serves as a marker for progenitor cells in human stomach.Methods and results: Immunohistochemistry revealed that Msi-1+ cells were detected especially in the isthmus/neck region (the putative position of stem cells) of the adult antrum, but were limited to the basal regions of fetal pyloric glands during the early stages of development. These results suggest that Msi-1 expression occurs specifically in the stem cell-containing regions. Msi-1+ cells were intermingled with proliferating cell muclear antigen (PCNA)+ cells in the isthmus/neck region of the adult antrum, but did not coexpress PCNA or Ki67. Msi-1 expression overlapped partly with expression of MUC5AC and MUC6, indicating that Msi-1+ cells retain some features of both foveolar and pyloric gland cell differentiation phenotypes. In contrast, Msi-1 expression in gastric glands showing intestinal metaplasia (IM) became weaker than that in the glands without IM.Conclusion: The specific expression of Msi-1 within the proliferative regions suggests that Msi-1 is a marker of cells with progenitor characteristics before active proliferation in human antrum.