Transplantation of mature adipocyte-derived dedifferentiated fat cells into three-wall defects in the rat periodontium induces tissue regeneration
Transplantation of mature adipocyte-derived dedifferentiated fat cells into three-wall defects in the rat periodontium induces tissue regeneration
复制标题
DOI：
10.2334/josnusd.16-0878
复制
发表时间：
2017-12-01
影响因子：
1.9
通讯作者：
Honda, Masaki
中科院分区：
文献类型：
--
作者：
Suzuki, Daigo;Akita, Daisuke;Honda, Masaki
去分化脂肪（DFAT）细胞与聚（d，l-乳酸-共-乙醇酸）（PLGA）支架组合的移植先前已被证明是促进大鼠开窗缺损模型中牙周组织再生的有效方法。本研究的目的是评估DFAT细胞在大鼠三壁牙周骨缺损模型中的再生潜力。在大鼠上颌第一磨牙的近中侧上双侧建立三壁骨缺损，并且不进行处理或通过植入含有DFAT细胞的PLGA支架或单独的PLGA进行处理。术后4周，对组织进行显微计算机断层扫描（micro-CT）和组织形态学检查。Micro-CT显示PLGA/DFAT组的骨再生率显著高于其他组，而组织形态计量学分析显示PLGA/DFAT组的脱细胞和细胞牙骨质中的胶原纤维束密度显著高于PLGA组。此外，结果表明，PLGA支架的放置防止了连接上皮的向下生长。这些结果表明，DFAT细胞有助于牙周三壁缺损的组织再生，而PLGA为牙周组织修复提供了必要的空间。
The transplantation of dedifferentiated fat (DFAT) cells in combination with poly(d,l-lactic-co-glycolic acid) (PLGA) scaffolds has previously been proven as an effective approach in promoting periodontal tissue regeneration in a rat fenestration defect model. The aim of this study was to assess the regenerative potential of DFAT cells in a rat model of three-wall periodontal bone defect. Three-wall bone defects were created bilaterally on the mesial side of rat maxillary first molars and were either left untreated or treated by implantation of PLGA scaffolds with DFAT cells or PLGA alone. Four weeks after surgery, the tissues were processed for micro-computed tomography (micro-CT) and histomorphometric examination. Micro-CT revealed that the PLGA/DFAT group had significantly higher rates of bone regeneration than the other groups, while histomorphometric analysis showed that the PLGA/DFAT group had significantly higher densities of collagen fiber bundles in acellular and cellular cementum than the PLGA group. Moreover, the results indicate that the placement of the PLGA scaffold prevented the downgrowth of the junctional epithelium. These findings suggest that DFAT cells contribute to tissue regeneration in three-wall periodontal defects, while PLGA provides space necessary for periodontal tissue restoration.