The specificity of the cAMP receptor mediating activation of adenylate cyclase in Dictyostelium discoideum.
The specificity of the cAMP receptor mediating activation of adenylate cyclase in Dictyostelium discoideum.
复制标题
盘基网柄菌中 cAMP 受体介导腺苷酸环化酶激活的特异性。
DOI：
10.1016/0012-1606(86)90216-2
复制
发表时间：
1986
影响因子：
2.7
通讯作者：
Devreotes,P
中科院分区：
文献类型：
--
作者：
Theibert,A;Palmisano,M;Jastorff,B;Devreotes,P
在盘状网柄藻中，环腺苷酸（cAMP）与表面受体的结合引起许多反应，包括趋化性、环GMP（cGMP）积累和腺苷酸环化酶的激活。通过测试一系列10种cAMP类似物的相对有效性来确定介导腺苷酸环化酶活化和cAMP分泌的表面cAMP受体的特异性。10种类似物中的每一种在相同的剂量范围内引起cAMP分泌、趋化性和cGMP积累。引发这些反应的效力顺序（cAMP > 2′-H-cAMP >N1-O-cAMP > cAMPS（Sp）> 6-Cl-cAMP > cAMPN（CH 3）2（Sp）> 3′-NH-cAMP > 8-Br-cAMP > cAMPS（Rp）> cAMPN（CH 3）2（Rp））与结合细胞表面主要cAMP结合位点的效力顺序相匹配，但与细胞表面磷酸二酯酶和主要细胞内cAMP结合蛋白的效力顺序不同。
InDictyostelium discoideumamoebae, binding of cyclic AMP (cAMP) to surface receptors elicits numerous responses including chemotaxis, cyclic GMP (cGMP) accumulation, and activation of adenylate cyclase. The specificity of the surface cAMP receptor which mediates activation of adenylate cyclase and cAMP secretion was determined by testing the relative effectiveness of a series of 10 cAMP analogs. Each of the 10 analogs elicited cAMP secretion, chemotaxis, and cGMP accumulation in the same dose range. The order of potency for eliciting these responses (cAMP > 2′-H-cAMP >N1-O-cAMP > cAMPS(Sp) > 6-Cl-cAMP > cAMPN(CH3)2(Sp) > 3′-NH-cAMP > 8-Br-cAMP > cAMPS(Rp) > cAMPN(CH3)2(Rp)) matches that for binding to the major cell surface cAMP binding sites and differs from that of the cell surface phosphodiesterase and the major intracellular cAMP binding protein.