A case study from the interaction of strawberry and Botrytis cinerea highlights the benefits of comonitoring both partners at genomic and mRNA level
A case study from the interaction of strawberry and Botrytis cinerea highlights the benefits of comonitoring both partners at genomic and mRNA level
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DOI：
10.1111/j.1469-8137.2005.01526.x
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发表时间：
2005-11-01
期刊：
影响因子：
9.4
通讯作者：
Hietala, AM
中科院分区：
文献类型：
--
作者：
Mehli, L;Kjellsen, TD;Hietala, AM
草莓Fragaria x ananassa（cv. Korona）接种灰葡萄孢（Botrytis cinerea），用实时荧光PCR、ELISA和麦角甾醇检测方法检测灰葡萄孢的定殖情况，其中第一种方法的灵敏度最高。利用实时荧光定量RT-PCR技术检测了病原菌β-微管蛋白和6种多聚半乳糖醛酸酶（Bcpg 1 -6）以及3种宿主防御基因（多聚半乳糖醛酸酶抑制蛋白（FaPGIP）和2种II类几丁质酶）的表达，发现宿主防御基因的最高转录水平出现在接种后16 h，即推测的入侵初期。Bcpg 2在96 h培养时间内的独特转录谱以及在8-24 hpi时相对于其他Bcpg的高转录水平表明该基因在穿透阶段具有特定的作用。转录谱的比较表明，Bcpg 1和Bcpg 3 -6协调调节。
Strawberry Fragaria x ananassa (cv. Korona) was inoculated with Botrytis cinerea by dipping berries in a conidial suspension.Colonization by the pathogen was monitored using real-time PCR, ELISA and ergosterol assays, the first showing the highest sensitivity. The expression of pathogen beta-tubulin and six polygalacturonases (Bcpg1-6) and three host defence genes (polygalacturonase-inhibiting protein (FaPGIP) and two class II chitinases) were monitored using real-time RT-PCR.The maximum transcript levels of the host defence genes occurred at 16 h postinoculation (hpi) at the presumed initial penetration stage. The unique transcript profile of Bcpg2 over the 96-h incubation time and its high transcript levels relative to those of the other Bcpgs at 8-24 hpi suggest that the gene has a specific role in the penetration stage.Bcpg1 was expressed constitutively at a relatively high level in actively growing mycelia throughout the experimental period. Comparison of the transcript profiles indicated that Bcpg1 and Bcpg3-6 were coordinately regulated.