Anti-glucosaminidase IgG in Sera as a biomarker of host immunity against Staphylococcus aureus in orthopaedic surgery patients.
Anti-glucosaminidase IgG in Sera as a biomarker of host immunity against Staphylococcus aureus in orthopaedic surgery patients.
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DOI：
10.2106/jbjs.l.01654
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Daiss, J. L.
中科院分区：
文献类型：
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作者：
Gedbjerg, N.;LaRosa, R.;Daiss, J. L.
背景：金黄色葡萄球菌感染仍然是骨科手术的主要并发症。虽然血清C-反应蛋白对诊断有用，但没有特异性的宿主免疫试验可以评估患者严重感染的风险。在确定氨基葡萄糖苷酶作为动物模型中的潜在保护性抗原的基础上，我们测试了以下假设：抗氨基葡萄糖苷酶IgG（免疫球蛋白G）水平在金黄色葡萄球菌感染的小鼠和骨科患者血清中存在差异，并且物理滴度和中和滴度相关。研究方法：开发了体外ELISA（酶联免疫吸附试验），以定量结合（物理）和酶中和（功能）抗氨基葡萄糖苷酶IgG滴度。使用来自幼稚、金黄色葡萄球菌攻击和氨基葡萄糖苷酶免疫小鼠的血清对测定进行验证。在24例骨科手术后确诊为金黄色葡萄球菌感染的患者和20例未感染患者的血清中测定了抗氨基葡萄糖苷酶IgG的物理、功能和同种型滴度。通过线性回归和受试者-操作者特征曲线分析评价抗氨基葡萄糖苷酶测定的特异性。结果如下：在小鼠中，抗氨基葡萄糖苷酶IgG的物理滴度测定的分析范围确定为1 ng/mL至1 g/mL，并且物理滴度与功能滴度相关（p
Background: Staphylococcus aureus infections remain a major complication of orthopaedic surgery. Although serum C-reactive protein is useful for diagnosis, there are no specific tests for host immunity that can assess a patient's risk for serious infection. On the basis of the identification of glucosaminidase as a potentially protective antigen in animal models, we tested the hypotheses that anti-glucosaminidase IgG (immunoglobulin G) levels vary in sera of mice and orthopaedic patients with Staphylococcus aureus infections and that physical and neutralizing titers correlate. Methods: In vitro ELISAs (enzyme-linked immunosorbent assays) were developed to quantify binding (physical) and enzyme-neutralizing (functional) anti-glucosaminidase IgG titers. The assays were validated with use of sera from naive, Staphylococcus aureus-challenged, and glucosaminidase-immunized mice. The physical, functional, and isotype titers of anti-glucosaminidase IgG were measured in sera from twenty-four patients with a confirmed Staphylococcus aureus infection following orthopaedic surgery and in sera from twenty noninfected patients. The specificity of the anti-glucosaminidase assay was evaluated by means of linear regression and receiver-operator characteristic curve analysis. Results: In mice, the analytic range of the physical titer assay for anti-glucosaminidase IgG was determined to be 1 ng/mL to 1 g/mL, and physical titers correlated with functional titers (p