ANTIGENIC SITES ON THE CVS RABIES VIRUS GLYCOPROTEIN - ANALYSIS WITH MONOCLONAL-ANTIBODIES
ANTIGENIC SITES ON THE CVS RABIES VIRUS GLYCOPROTEIN - ANALYSIS WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1099/0022-1317-64-4-843
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发表时间：
1983-01-01
影响因子：
3.8
通讯作者：
MACFARLAN, RI
中科院分区：
文献类型：
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作者：
LAFON, M;WIKTOR, TJ;MACFARLAN, RI
本文研究了狂犬病病毒（CVS-11）糖蛋白的抗原性变异。在抗糖蛋白单克隆抗体存在下，体外以高频率（10-4-10-5）分离出耐中和变异病毒。基于不再被≥中和的变体的分组，这些变体的分析鉴定出至少3个功能独立的抗原位点。24种单克隆抗体中的1种。竞争放射免疫分析表明，这2个抗原位点之一是拓扑不同，与其他2个在密切的接近。结果表明，大多数（但不是全部）中和抗性变体未能结合相关单克隆抗体。在不存在抗体的情况下，在体外几次传代后，显示抗原性改变的病毒在病毒储备液中蓄积。在用单克隆抗体处理小鼠后在体内分离变体。
Antigenic variation in the glycoprotein of rabies (CVS-11) virus was studied. Neutralization-resistant variant viruses were isolated in vitro at high frequency (10-4-10-5) in the presence of anti-glycoprotein monoclonal antibody. Analysis of these variants identified at least 3 functionally independent antigenic sites, based on the grouping of variants that were no longer neutralized by .gtoreq. 1 of a panel of 24 monoclonal antibodies. Competition radioimmunoassay suggested that one of these 2 antigenic sites was topologically distinct, with the other 2 in close proximity. It was shown that most (but not all) neutralization-resistant variants failed to bind the relevant monoclonal antibody. Viruses with altered antigenicity were shown to accumulate in virus stocks following several passages in vitro in the absence of antibody. Variants were isolated in vivo following treatment of mice with monoclonal antibody.