Context-dependent photodimerization in isolated thymine-thymine steps in DNA
Context-dependent photodimerization in isolated thymine-thymine steps in DNA
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DOI：
10.1021/ja804950j
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发表时间：
2008-09-10
影响因子：
15
通讯作者：
Lewis, Frederick D.
中科院分区：
文献类型：
--
作者：
Hariharan, Mahesh;Lewis, Frederick D.
研究了280 nm照射对一个合成DNA发夹家族的影响，该家族具有连接六个碱基对茎的烷烃接头，该茎具有位于发夹内不同位置的单个T-T步骤。得到了一个2+2二聚产物的加合物，除了在连接体附近有一个T-T步骤的情况，在这种情况下，得到了2+2和6 - 4加合物。二聚化的效率对于具有位于双链体内部的T-T步骤的三个发夹是相似的。对于位于发夹的开放末端和T突出端中的T-T步骤，观察到较低的效率，而对于邻近接头和单个T凸起中的T-T步骤，观察到较高的效率。讨论了二聚效率的上下文依赖性。
The effect of 280 nm irradiation on a family of synthetic DNA hairpins possessing an alkane linker connecting a six-base pair stem having a single T−T step located at different positions within the hairpin has been investigated. A single adduct assigned to the product of 2+2 dimerization is obtained except in the case of a T−T step located adjacent to the linker, in which case both 2+2 and 6−4 adducts are obtained. The efficiency of dimerization is similar for three hairpins having a T−T step located within the duplex interior. Lower efficiency is observed for a T−T step located at the open end of the hairpin and in T overhangs, whereas higher efficiency is observed for the T−T step adjacent to the linker and in a single T bulge. The context-dependence of dimerization efficiency is discussed.