Characterization of a highly polymeric proanthocyanidin fraction from persimmon pulp with strong Chinese cobra PLA2 inhibition effects
Characterization of a highly polymeric proanthocyanidin fraction from persimmon pulp with strong Chinese cobra PLA2 inhibition effects
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DOI：
10.1016/j.fitote.2011.10.005
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发表时间：
2012-01-01
期刊：
影响因子：
3.4
通讯作者：
Li, Chun-mei
中科院分区：
文献类型：
--
作者：
Xu, Shu-fen;Zou, Bo;Li, Chun-mei
用TOYOPEL TSK-HW-50-F对柿原花青素进行了分级，得到了对中国眼镜蛇解放军的催化活性、致水活性和致死性有很强抑制作用的组分(2)。硫解分析表明，末端单元包括C.EGCG和杨梅素，表儿茶素、表没食子儿茶素、(Epi)没食子儿茶素-3-O-没食子酸酯和(Epi)儿茶素-3-O-没食子酸酯是延伸单元。平均聚合度为23.7。MALDI TOF/MS、硫代高效液相、FTIR和圆二色谱(CD)分析表明，该组分具有较高的前飞蓬含量(55%)和很高的3-O-没食子酸化程度(92%)。它以A型键为主，具有黄烷基的4个β键和4R绝对构型。(C)2011爱思唯尔B.V.保留所有权利。
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C. EGCG and myricetin, and epicatechin, epigallocatechin, (epi)gallocatechin-3-O-gallate, and (epi)catechin-3-O-gallate occurred as extender units. The mean degree of polymerization was 23.7. MALDI TOF/MS, thioly-HPLC, FTIR and circular dichroism (CD) analyses showed that the fraction had high prodelphinidin content (55%) and a very high degree of 3-O-galloylation (92%). A type linkage is dominant in it and it had 4 beta linkage of the flavanyl substituent and 4R absolute configuration. (C) 2011 Elsevier B.V. All rights reserved.