Bond-Strengthening in Staphylococcal Adhesion to Hydrophilic and Hydrophobic Surfaces Using Atomic Force Microscopy
Bond-Strengthening in Staphylococcal Adhesion to Hydrophilic and Hydrophobic Surfaces Using Atomic Force Microscopy
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DOI：
10.1021/la801824c
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发表时间：
2008-11-18
期刊：
影响因子：
3.9
通讯作者：
Norde, Willem
中科院分区：
文献类型：
--
作者：
Boks, Niels P.;Busscher, Henk J.;Norde, Willem
研究了4株表皮葡萄球菌对疏水和亲水表面的时间依赖性细菌粘附力。两个表面之间的初始粘附力显著不同，并且徘徊在-0.4nN左右。基质表面疏水性对四种不同S.表皮菌株。随着时间的推移，疏水二甲基二氯硅烷（DDS）涂覆的玻璃上几乎不存在粘合力的增强，尽管在少数情况下，在回缩曲线中出现了多个粘合峰。亲水性玻璃上的粘合强化发生在5-35 s内，最大粘合力为-1.9 +/-0.7 nN，并且在回缩时同时出现多个粘合峰。多个粘附峰的泊松分析允许将氢键的贡献与其他非特异性相互作用力分离，并揭示氢键的作用力贡献为-0.8 nN，其他非特异性相互作用力的作用力贡献为+0.3 nN。所有四种葡萄球菌菌株的时间依赖性细菌粘附力相当。它的结论是，在DDS涂层的玻璃，疏水效应引起瞬时粘附，而加强的债券亲水性玻璃上的主要是由非瞬时氢键的形成。
Time-dependent bacterial adhesion forces of four strains of Staphylococcus epidermidis to hydrophobic and hydrophilic surfaces were investigated. Initial adhesion forces differed significantly between the two surfaces and hovered around -0.4 nN. No unambiguous effect of substratum surface hydrophobicity on initial adhesion forces for the four different S. epidermidis strains was observed. Over time, strengthening of the adhesion forces was virtually absent on hydrophobic dimethyldichlorosilane (DDS)-coated glass, although in a few cases multiple adhesion peaks developed in the retract curves. Bond-strengthening on hydrophilic glass occurred within 5-35 s to maximum adhesion forces of -1.9 +/- 0.7 nN and was concurrent with the development of multiple adhesion peaks upon retract. Poisson analysis of the multiple adhesion peaks allowed separation of contributions of hydrogen bonding from other nonspecific interaction forces and revealed a force contribution of -0.8 nN for hydrogen bonding and +0.3 nN for other nonspecific interaction forces. Time-dependent bacterial adhesion forces were comparable for all four staphylococcal strains. It is concluded that, on DDS-coated glass, the hydrophobic effect causes instantaneous adhesion, while strengthening of the bonds on hydrophilic glass is dominated by noninstantaneous hydrogen bond formation.