Silver-Zinc Redox-Coupled Electroceutical Wound Dressing Disrupts Bacterial Biofilm
Silver-Zinc Redox-Coupled Electroceutical Wound Dressing Disrupts Bacterial Biofilm
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DOI：
10.1371/journal.pone.0119531
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发表时间：
2015-03-24
期刊：
影响因子：
3.7
通讯作者：
Sen, Chandan K.
中科院分区：
文献类型：
--
作者：
Banerjee, Jaideep;Das Ghatak, Piya;Sen, Chandan K.
铜绿假单胞菌生物被膜通常与慢性伤口感染相关。研究了FDA批准的无线电细胞敷料（WED）的抗生物膜性能，该敷料在存在导电伤口渗出物的情况下被激活以产生电场（0.3-0.9V）。在WED的存在下，致病性铜绿假单胞菌菌株PAO 1在LB培养基中的生长被显著抑制。扫描电子显微镜显示，在银敷料无效的情况下，WED明显破坏了生物膜的完整性。在WED的存在下，生物膜厚度和活细菌细胞的数量减少。群体感应基因lasR和rhlR以及电场敏感酶甘油-3-磷酸脱氢酶的活性也被WED抑制。这项工作提供了第一个电子顺磁共振光谱证据表明，WED作为一个自发源的活性氧物种。氧化还原敏感的多药外排系统mexAB和mexEF被WED抑制。总之，这些观察结果提供了支持WED抗生物膜特性的第一个证据。
Pseudomonas aeruginosa biofilm is commonly associated with chronic wound infection. A FDA approved wireless electroceutical dressing (WED), which in the presence of conductive wound exudate gets activated to generate electric field (0.3-0.9V), was investigated for its anti-biofilm properties. Growth of pathogenic P. aeruginosa strain PAO1 in LB media was markedly arrested in the presence of the WED. Scanning electron microscopy demonstrated that WED markedly disrupted biofilm integrity in a setting where silver dressing was ineffective. Biofilm thickness and number of live bacterial cells were decreased in the presence of WED. Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED. This work provides first electron paramagnetic resonance spectroscopy evidence demonstrating that WED serves as a spontaneous source of reactive oxygen species. Redox-sensitive multidrug efflux systems mexAB and mexEF were repressed by WED. Taken together, these observations provide first evidence supporting the anti-biofilm properties of WED.