Duplicate US1 Genes of Duck Enteritis Virus Encode a Non-essential Immediate Early Protein Localized to the Nucleus
Duplicate US1 Genes of Duck Enteritis Virus Encode a Non-essential Immediate Early Protein Localized to the Nucleus
复制标题
鸭肠炎病毒的重复 US1 基因编码定位于细胞核的非必需立即早期蛋白
DOI：
10.3389/fcimb.2019.00463
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发表时间：
2020-01-17
影响因子：
5.7
通讯作者：
Cheng, Anchun
中科院分区：
文献类型：
--
作者：
Li, Yangguang;Wu, Ying;Cheng, Anchun
鸭肠炎病毒（DEV）的US 1基因编码一个保守的Herpes_IE68结构域，该结构域与疱疹病毒立即早期调节蛋白家族密切相关，在Herpes_IE68基因编码的蛋白中高度保守。先前的研究发现同源蛋白HSV-1 ICP 22和VZV ORF 63/ORF 70对病毒的转录和复制至关重要。然而，对DEV ICP 22蛋白知之甚少。在本文中，我们描述了该蛋白的药理实验，实时定量聚合酶链反应，蛋白质印迹和免疫荧光测定的基础上的特性。我们还研究了通过US 1突变的蛋白质在DEV复制中的作用。结果，我们发现DEV ICP 22蛋白是一种非必需的立即早期蛋白，主要位于感染的DEF细胞的细胞核中，并且DEV复制因US 1缺失而受损。我们还发现ICP 22在305- 312位氨基酸处含有一个经典的核定位信号（NLS），并且ICP 22在突变309位残基后不能自行进入细胞核。
The duplicate US1 genes of duck enteritis virus (DEV) encode a protein with a conserved Herpes_IE68 domain, which was found to be closely related to the herpes virus immediate early regulatory protein family and is highly conserved among counterparts encoded by Herpes_IE68 genes. Previous studies found the homologous proteins HSV-1 ICP22 and VZV ORF63/ORF70 to be critical for virus transcription and replication. However, little is known about the DEV ICP22 protein. In this paper, we describe the characteristics of this protein based on pharmacological experiments, real-time quantitative Polymerase Chain Reaction, Western blot, and immunofluorescence assays. We also investigate the role of the protein in DEV replication via mutation of US1. As a result, we found that the DEV ICP22 protein is a non-essential immediate early protein predominantly located in the nucleus of infected DEF cells and that DEV replication is impaired by US1 deletion. We also found that ICP22 contains a classical nuclear localization signal (NLS) at 305-312AA, and ICP22 cannot enter the nucleus by itself after mutating residue 309.