Genes for the hook-basal body proteins of the flagellar apparatus in Escherichia coli
Genes for the hook-basal body proteins of the flagellar apparatus in Escherichia coli
复制标题
大肠杆菌鞭毛器钩基体蛋白的基因
作者：
Y. Komeda;M. Silverman;P. Matsumura;M. Simon
在鞭毛功能所需的30多个基因中，有6个位于大肠杆菌基因组pyrC和ptsG之间，这一基因簇被称为鞭毛区I。四点转导杂交用于建立相对于外部标记ptsG和pyrC的区域I鞭毛基因的位置和顺序。噬菌体E.构建了含有鞭毛形成所必需的大部分基因的大肠杆菌杂交体。携带区域I弗拉基因的特定杂交体的性质通过遗传互补和通过测量杂交体指导特定多肽合成的能力来检查。这些测试的结果与λ杂交和与一系列缺失突变来自λ杂交证明存在至少六个鞭毛特异性顺反子。这些指导了具有以下表观分子量的多肽的合成：flaV，11，000; flaK，42，000; flaL，30，000和27，000; flaM，38，000; flS，60，000;和flaT，35，000。质粒ColE 1-E.具有I区鞭毛基因的大肠杆菌杂合体也用于在产生小细胞的菌株中编程多肽的合成。在这些实验中合成的多肽与钩-基体结构的多肽相同，并有助于确认基因对特定多肽的分配。所有这些多肽的合成都受到调节其他鞭毛相关结构成分合成的相同机制的调节。
Of the more than 30 genes required for flagellar function, 6 are located between pyrC and ptsG on the Escherichia coli genetic man. This cluster of genes is called flagellar region I. Four-point transductional crosses were used to establish the position and order of the region I flagellar genes with respect to the outside markers ptsG and pyrC. Bacteriophage lambda-E. coli hybrids that contained most of the genes necessary for flagellar formation were constructed. The properties of specific hybrids that carried the region I fla genes were examined by genetic complementation and by measuring the capacity of the hybrids to direct the synthesis of specific polypeptides. The results of these tests with lambda hybrids and with a series of deletion mutations derived from the lambda hybrids demonstrated the existence of at least six flagellar-specific cistrons. These directed the synthesis of polypeptides with the following apparent molecular weights: flaV, 11,000; flaK, 42,000; flaL, 30,000 and 27,000; flaM, 38,000; flS, 60,000; and flaT, 35,000. Plasmid ColE1-E. coli hybrids with region I flagellar genes were also used to program the synthesis of polypeptides in minicell-producing strains. The polypeptides synthesized in these experiments were identical to polypeptides of the hook-basal body structure and helped to confirm the assignment of genes to specific polypeptides. The synthesis of all of these polypeptides was regulated by the same mechanism that regulates the synthesis of other flagellar-related structural components.