Adipocyte differentiation selectively represses the serum inducibility of c-jun and junB by reversible transcription-dependent mechanisms.
Adipocyte differentiation selectively represses the serum inducibility of c-jun and junB by reversible transcription-dependent mechanisms.
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脂肪细胞分化通过可逆转录依赖性机制选择性抑制 c-jun 和 junB 的血清诱导能力。
DOI：
10.1073/pnas.91.11.4649
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发表时间：
1994
影响因子：
11.1
通讯作者：
Scott,RE
中科院分区：
文献类型：
--
作者：
Wang,H;Scott,RE
非终末分化的 3T3 T 脂肪细胞对 10% (vol/vol) 胎牛血清的生长刺激具有抵抗力，尽管它们可以用极高的血清浓度诱导增殖。我们现在报道，在脂肪细胞中，10% 胎牛血清通常也不能诱导 c-jun 或 junB。相反，在 10% 胎牛血清处理后，c-jun 和 junB 表达在短暂的初始轻微诱导后明显受到抑制。凝胶迁移率变化研究证实 AP-1 DNA 结合活性在脂肪细胞中受到抑制。脂肪细胞中 c-jun 和 junB 诱导性的抑制是由转录机制引起的，可以通过蛋白质合成抑制剂或更高血清浓度的治疗来逆转，并且不影响 c-fos 或 c-myc 表达。这些数据表明，脂肪细胞分化选择性地和转录地抑制c-jun和junB的诱导能力，从而降低细胞响应10%胎牛血清的增殖能力。
Nonterminally differentiated 3T3 T adipocytes are resistant to growth stimulation by 10% (vol/vol) fetal bovine serum even though they can be induced to proliferate with extremely high serum concentrations. We now report that in adipocytes 10% fetal bovine serum also fails to typically induce c-jun or junB. Rather, after 10% fetal bovine serum treatment, c-jun and junB expression is markedly repressed after a brief initial slight induction. Gel mobility shift studies confirm that AP-1 DNA binding activity is inhibited in adipocytes. Repression in c-jun and junB inducibility in adipocytes results from transcriptional mechanisms, can be reversed by treatment with protein synthesis inhibitors or higher serum concentrations, and does not affect c-fos or c-myc expression. These data suggest that adipocyte differentiation selectively and transcriptionally represses the inducibility of c-jun and junB so as to decrease the cell's ability to proliferate in response to 10% fetal bovine serum.