Identification of multiple transcription factor genes potentially involved in the development of electrosensory versus mechanosensory lateral line organs
Identification of multiple transcription factor genes potentially involved in the development of electrosensory versus mechanosensory lateral line organs
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DOI：
10.1101/2023.04.14.536701
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发表时间：
2023-09
期刊：
影响因子：
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通讯作者：
Martin Minařík;Melinda S. Modrell;J. Andrew Gillis;Alexander S. Campbell;Isobel Fuller;R. Lyne;G. Micklem;D. Gela;M. Pšenička;Clare V. H. Baker
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作者：
Martin Minařík;Melinda S. Modrell;J. Andrew Gillis;Alexander S. Campbell;Isobel Fuller;R. Lyne;G. Micklem;D. Gela;M. Pšenička;Clare V. H. Baker
在电感受性颌骨脊椎动物中，胚胎侧线胎盘形成电感觉壶腹部器官和机械感觉神经肥大。先前关于共享基因表达的报道表明，电感受器和机械感觉毛细胞发育的基础是保守的机制，并且电感受器进化为与毛细胞转录相关的姐妹细胞类型。我们之前只发现了一个转录因子基因，Neurod4，它是密西西比白鳍鱼(Polyodon Spathula)的壶腹器官受限于发育中的侧线系统的软骨鱼。我们之前在白鳍鱼中验证的其他16个转录因子基因在壶腹器官和神经肥大中都有表达。在这里，我们使用我们发表的侧线器官丰富基因集(来自晚期仔鱼的差异整体RNA-seq)，结合候选基因方法，鉴定了23个转录因子基因，这些基因在更容易实验驯化的软骨类动物(小鲟鱼)和/或白鳍鱼的侧线系统中表达。12在壶腹器官和神经肥大细胞中均有表达，这与分子机制的保守性一致。其中6个是头部的电感觉受限基因(IRX5、Insm1、SP5、Satb2、MafA和RORC)，5个是首次报道的机械感觉受限转录因子基因(FOXG1、Sox8、Isl1、Hmx2和Rob)。然而，正如以前报道的那样，Sox8在鲨鱼的壶腹器官和神经肥大中都有表达，这表明软骨鱼和鳍鱼之间存在特定的谱系差异。总体而言，我们的结果支持壶腹器官和神经肥大通过高度保守的转录机制发育的假说，并确定了可能参与电感觉和机械感觉侧线器官形成的多种转录因子。
In electroreceptive jawed vertebrates, embryonic lateral line placodes give rise to electrosensory ampullary organs as well as mechanosensory neuromasts. Previous reports of shared gene expression suggest that conserved mechanisms underlie electroreceptor and mechanosensory hair cell development and that electroreceptors evolved as a transcriptionally related ’sister cell type’ to hair cells. We previously identified only one transcription factor gene, Neurod4, as ampullary organ-restricted in the developing lateral line system of a chondrostean ray-finned fish, the Mississippi paddlefish (Polyodon spathula). The other 16 transcription factor genes we previously validated in paddlefish were expressed in both ampullary organs and neuromasts. Here, we used our published lateral line organ-enriched gene-set (arising from differential bulk RNA-seq in late-larval paddlefish), together with a candidate gene approach, to identify 23 transcription factor genes expressed in the developing lateral line system of a more experimentally tractable chondrostean, the sterlet (Acipenser ruthenus, a small sturgeon), and/or that of paddlefish. Twelve are expressed in both ampullary organs and neuromasts, consistent with conservation of molecular mechanisms. Six are electrosensory-restricted on the head (Irx5, Insm1, Sp5, Satb2, MafA and Rorc), and five are the first-reported mechanosensory-restricted transcription factor genes (Foxg1, Sox8, Isl1, Hmx2 and Rorb). However, as previously reported, Sox8 is expressed in ampullary organs as well as neuromasts in a shark (Scyliorhinus canicula), suggesting the existence of lineage-specific differences between cartilaginous and ray-finned fishes. Overall, our results support the hypothesis that ampullary organs and neuromasts develop via largely conserved transcriptional mechanisms, and identify multiple transcription factors potentially involved in the formation of electrosensory versus mechanosensory lateral line organs.