Dynamic gene expression during the onset of myoblast differentiation in vitro
Dynamic gene expression during the onset of myoblast differentiation in vitro
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DOI：
10.1016/s0888-7543(03)00104-6
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发表时间：
2003-08-01
期刊：
影响因子：
4.4
通讯作者：
Zhang, PM
中科院分区：
文献类型：
--
作者：
Delgado, I;Huang, XX;Zhang, PM
骨骼肌发生是一个研究充分的分化过程。然而，尽管鉴定和功能特性的肌源性基本HLH转录因子，分子的细节仍然缺乏。随着微阵列技术的出现，以前所未有的规模观察生物过程中基因表达谱的变化成为可能。在这项研究中，我们应用这项技术，在体外分化的成肌细胞系，C2C12的基因表达谱。我们报告了超过1500个基因的表达改变时，这些细胞分化，包括624个，或约40%的基因总数，与未知的功能。该分析揭示了存在12组协调调节的基因，其在肌生成素的转录诱导之前以基因表达的时间波表达。其中有多个家族的转录因子是重要的过程中的肌。此外，Notch信号通路的诱导表明，以前未被重视的细胞间信号传导发生在肌源性分化过程中。这些结果提供了骨骼肌发生直到肌细胞生成素激活的分子描述。(C)2003 Elsevier Science（美国）。All rights reserved.
Skeletal myogenesis is a well-studied differentiation process. However, despite the identification and functional characterization of the myogenic basic HLH transcription factors, molecular details are still lacking. With the advent of microarray technology, it has become possible to look at changes in gene expression profiles in a biological process on an unprecedented scale. In this study, we applied this technology to profile gene expression during the in vitro differentiation of an established myoblast cell line, C2C12. We report over 1500 genes whose expression is altered when these cells differentiate, including 624, or about 40% of the total number of genes, with unknown functions. This analysis reveals the existence of 12 groups of coordinately regulated genes that are expressed in temporal waves of gene expression prior to the transcriptional induction of myogenin. Among these are multiple families of transcription factors that are important for the process of myogenesis. In addition, the induction of the Notch signaling pathway suggests that previously unappreciated intercellular signaling occurs during myogenic differentiation. These results provide a molecular description of the skeletal myogenesis up to the activation of myogenin. (C) 2003 Elsevier Science (USA). All rights reserved.