Gold Nanoparticles: Microbial Synthesis and Application in Water Hygiene Management
Gold Nanoparticles: Microbial Synthesis and Application in Water Hygiene Management
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DOI：
10.1021/la900585p
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发表时间：
2009-07-21
期刊：
影响因子：
3.9
通讯作者：
Guha, Arun K.
中科院分区：
文献类型：
--
作者：
Das, Sujoy K.;Das, Akhil R.;Guha, Arun K.
介绍了一种绿色化学合成纳米金-生物复合物的方法及其对污染沃茨的净化作用。通过原位还原氯金酸（HAuCl 4），在根霉（Rhizopus）表面制备了平均直径为10 nm的金纳米粒子。纳米金-生物复合物（NGBC）对多种有机磷农药表现出较强的吸附能力。EDXA研究证实了农药在缀合物材料表面上的吸附。用原子力显微镜观察了NGBC材料吸附有机磷农药后的形态变化。NGBC对几种革兰氏阴性和革兰氏阳性病原菌以及酵母菌酿酒酵母和白色念珠菌显示出高的抗微生物活性。用NGBC处理微生物细胞引起细胞膜破裂，如扫描电子和荧光显微照片所示。NGBC的这些独特特性已被成功地用于在一次操作中获得不含病原体和杀虫剂的饮用水。
A green chemical method to synthesize nanogold-bioconjugate and its eco-friendly promising role to purify contaminated waters has been described. Gold nanoparticles of 10 nm average diameter are produced on the surface of Rhizopus oryzae, a fungal strain, by in situ reduction of chloroauric acid (HAuCl4). The nanogold-bioconjugate (NGBC) showed strong adsorption capacity toward different organophosphorous pesticides. The EDXA study confirms adsorption of pesticides on the conjugate material surface. Morphological changes of the NGBC material after adsorption of organophosphorous pesticides were detected by atomic force micrographs. NGBC shows high antimicrobial activity against several Grain-negative and Gram-positive pathogenic bacteria as well as the yeasts Saccharomyces cerevisiae and Candida albicans. The treatment of microbial cells with NGBC caused rupture of cell membrane as revealed in scanning electron and fluorescence micrographs. These unique characteristics of NGBC have been successfully utilized to obtain potable water free from pathogens and pesticides in a single operation.