Effect of heat stress on contractility of tissue-engineered artificial skeletal muscle
Effect of heat stress on contractility of tissue-engineered artificial skeletal muscle
复制标题
热应激对组织工程人工骨骼肌收缩性的影响
DOI：
10.1007/s10047-018-1020-y
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发表时间：
2018
影响因子：
1.3
通讯作者：
Fujisato Toshia
中科院分区：
文献类型：
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作者：
Takagi Shunya;Nakamura Tomohiro;Fujisato Toshia
热应激对骨骼肌等组织的影响已被广泛研究。然而，负责热应激的影响的机制仍然不清楚。一个有用的实验组织模型是必要的，因为在细胞培养的肌肉功能可能不同于天然肌肉和测量其收缩性是困难的。我们先前报道了三维组织工程化人工骨骼肌（TEM），可以很容易地设置在测量装置的收缩力的定量评价。我们现在已将透射电镜应用于热应力的研究。我们在39 °C下热暴露24或48小时后立即分析收缩性以评估急性效应，并在热暴露后进行正常培养以评估后效。收缩力峰值和收缩峰值时间被用作收缩参数。正常培养后早期（1周）热应激使TCF增加，中后期（2-3周）TCF暂时下降。这些结果表明，热应激可能影响TEM培养早期的成肌细胞融合和肌管分化，但不影响后期的肌管成熟。热暴露组TCF增加率显著高于无热暴露组。虽然在分子水平上的详细分析是必要的进一步调查，我们的人工骨骼肌可能是一个有前途的工具，热应力调查。
The effects of heat stress on tissue like skeletal muscle have been widely studied. However, the mechanism responsible for the effect of heat stress is still unclear. A useful experimental tissue model is necessary because muscle function in cell culture may differ from native muscle and measuring its contractility is difficult. We previously reported three-dimensional tissue-engineered artificial skeletal muscle (TEM) that can be easily set in a measurement apparatus for quantitative evaluation of contractility. We have now applied TEM to the investigation of heat stress. We analyzed contractility immediately after thermal exposure at 39 °C for 24 or 48 h to evaluate the acute effects and after thermal exposure followed by normal culture to evaluate the aftereffects. Peak twitch contractile force and time-to-peak twitch were used as contractile parameters. Heat stress increased the TCF in the early stage (1 week) after normal culture; the TCF decreased temporarily in the middle to late stages (2–3 weeks). These results suggest that heat stress may affect both myoblast fusion and myotube differentiation in the early stage of TEM culture, but not myotube maturation in the late stage. The TCF increase rate with thermal exposure was significantly higher than that without thermal exposure. Although detailed analysis at the molecular level is necessary for further investigation, our artificial skeletal muscle may be a promising tool for heat stress investigation.