Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system
Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system
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DOI：
10.1161/01.res.83.10.980
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发表时间：
1998-11-16
影响因子：
20.1
通讯作者：
Lee, ME
中科院分区：
文献类型：
--
作者：
Jain, MK;Kashiki, S;Lee, ME
LIM家族的蛋白质是各种细胞类型中发育和分化的关键调节剂。我们已经描述了富含半胱氨酸的蛋白2/平滑肌LIM蛋白（CRP 2/SmLIM），一个LIM只在分化的血管平滑肌细胞中表达的蛋白的克隆。作为了解CRP 2/SmLIM潜在功能的第一步，我们分析了其在发育小鼠原肠胚形成后的表达，并将CRP 2/SmLIM的表达与CRP亚类的其他2个成员CRP 1和CRP 3/MLP的表达进行了比较。CRP 2/SmLIM在胚胎第10天开始在血管中表达，在随后的时间点，CRP 2/SmLIM在心脏中的表达下降，但在血管中的表达仍然很高，CRP 1在含有平滑肌细胞的血管和非血管组织中均有表达，而CRP 3/MLP仅在含有横纹肌的组织中表达。这些表达模式在成年动物中得到维持，并表明该基因家族在平滑肌和横纹肌的发育中起重要作用。
Proteins of the LIM family are critical regulators of development and differentiation in various cell types. We have described the cloning of cysteine-rich protein 2/smooth muscle LIM protein (CRP2/SmLIM), a LIM-only protein expressed in differentiated vascular smooth muscle cells. As a first step toward understanding the potential functions of CRP2/SmLIM, we analyzed its expression after gastrulation in developing mice and compared the expression of CRP2/SmLIM with that of the other 2 members of the CRP subclass, CRP1 and CRP3/MLP, In situ hybridization in whole-mount and sectioned embryos showed that CRP2/StnLIM was expressed in the sinus venosus and the 2 cardiac chambers at embryonic day 9. Vascular expression of CRP2/SmLIM was first seen at embryonic day 10, At subsequent time points, CRP2/SmLIM expression decreased in the heart but remained high in the vasculature, CRP1 was expressed both in vascular and nonvascular tissues containing smooth muscle cells, whereas CRP3/MLP was expressed only in tissues containing striated muscle. These patterns of expression were maintained in the adult animal and suggest an important role for this gene family in the development of smooth and striated muscle.