Hepatitis C RNA in liver of chronic hepatitis C patients before and after interferon alfa treatment.
Hepatitis C RNA in liver of chronic hepatitis C patients before and after interferon alfa treatment.
复制标题
干扰素α治疗前后慢性丙型肝炎患者肝脏中的丙型肝炎RNA。
DOI：
10.1016/0016-5085(93)90358-j
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发表时间：
1993
期刊：
影响因子：
29.4
通讯作者：
Gerber,MA
中科院分区：
文献类型：
--
作者：
Balart,LA;Perrillo,R;Roddenberry,J;Regenstein,F;Shim,KS;Shieh,YS;Taylor,B;Dash,S;Gerber,MA
背景：干扰素α成功治疗慢性丙型肝炎后，往往会复发。由于血清中丙型肝炎病毒RNA(HCV-RNA)的丢失不能预测持续应答，因此我们研究了肝脏中的HCV-RNA丢失作为持续应答的预测指标。方法：对21例接受重组干扰素α治疗的慢性丙型肝炎患者，在治疗前后和治疗结束时分别检测冰冻肝组织和血清中的丙型肝炎病毒RNA序列。结果：11例完全应答者中有10例(91%)在肝脏中检测到了丙型肝炎病毒RNA，而在8例无应答者中有7例(87%)在肝脏或血清中仍可检测到。在6个月的随访中，5名完全应答者生化复发；其中4名在治疗结束时在肝脏中未检测到丙型肝炎病毒RNA。结论：肝脏中丙型肝炎病毒RNA的消失与最初的临床结果相关，但正如先前报道的血清丙型肝炎病毒RNA一样，这种丢失并不一定能预测持续应答。
Background:Successful treatment of chronic hepatitis C with interferon alfa is frequently followed by relapse. Because loss of hepatitis C viral RNA (HCV-RNA) in serum is not predictive of sustained response, the loss of HCV-RNA in liver as a predictor of sustained response was investigated.Methods:Twenty-one patients with chronic hepatitis C treated with recombinant interferon alfa had HCV-RNA sequences determined in frozen liver tissue before and after treatment and in serum at the end of treatment. Reverse double polymerase chain reaction was used to detect sequences to the 5′ nontranslated region of the HCV genome using double nested primers.Results:HCV-RNA disappeared in the liver in 10 of 11 (91%) complete responders whereas it remained detectable in the liver or serum of 7 of 8 (87%) nonresponders. Five complete responders relapsed biochemically during 6 month's follow-up; 4 of these had no detectable HCV-RNA in liver at end of treatment.Conclusions:Disappearance of HCV-RNA in liver correlates with initial clinical outcome, but as previously reported with serum HCV-RNA, this loss does not necessarily allow prediction of a sustained response.