STRUCTURE OF TRICHORZIANINE-A IIIC, AN ANTIFUNGAL PEPTIDE FROM TRICHODERMA-HARZIANUM
STRUCTURE OF TRICHORZIANINE-A IIIC, AN ANTIFUNGAL PEPTIDE FROM TRICHODERMA-HARZIANUM
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DOI：
10.1021/ja00307a028
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发表时间：
1985-01-01
影响因子：
15
通讯作者：
DAVOUST, D
中科院分区：
文献类型：
--
作者：
BODO, B;REBUFFAT, S;DAVOUST, D
TricHorianine A IIIc是从哈茨木霉孢子化培养物中分离出来的抑制某些真菌病原体生长的多肽混合物的主要成分。这种新的多肽由一个乙酰化的N-末端残基、18个氨基酸和一个色氨酸C-末端的氨基醇组成。序列指认是基于正离子FAB质谱学和高场核磁共振数据，使用二维核磁共振指配技术和质子NOE差异研究。有关构象的信息由JNH-CαH偶合常数、溶剂和温度与NH化学位移的关系以及溶剂饱和转移实验获得。这些数据显示该肽的N-末端部分以螺旋形式排序，其第一个转角是310型。
Trichorizianine A IIIc is the main component of a peptide mixture isolated from a sporulated culture of Trichoderma harzianum that inhibits the growth of some fungal pathogens. This novel peptide has been shown to consist of one acetylated N-terminal residue, 18 amino acids, and a tryptophanol C-terminal amino alcohol. Sequence assignment was based on positive-ion FAB mass spectrometry and high-field NMR data using two-dimensional NMR assignment techniques and proton NOE difference studies. Information on the conformation was derived from JNH-C.alpha.H coupling constants, solvent and temperature NH chemical shift dependence, and transfer of solvent saturation experiments. These data show the N-terminal part of the peptide to be ordered in a helix, the first turn of which is of the 310 type.