Mitochondrial genomes of the Dorcus velutinus complex (Coleoptera: Lucanidae) with the large intergenic spacer showing unique short sequence repeats and their implications for systematics
Mitochondrial genomes of the Dorcus velutinus complex (Coleoptera: Lucanidae) with the large intergenic spacer showing unique short sequence repeats and their implications for systematics
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带有大基因间间隔区的 Dorcus velutinus 复合体（鞘翅目：Lucanidae）的线粒体基因组显示出独特的短序列重复及其对系统学的影响
DOI：
10.1016/j.aspen.2020.08.015
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发表时间：
2021-04-12
影响因子：
1.5
通讯作者：
Wan, Xia
中科院分区：
文献类型：
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作者：
Chen, Yong-Jing;Kim, Sangil;Wan, Xia
利用下一代测序技术，对鹿角线虫（Dorcus velutinus Thomson）、D. ursulus Arrow和D. tenuihirsutus Kim和Kim这3个鹿角线虫物种的线粒体基因组进行了组装和分析。有丝分裂基因组序列被用来推断Dorcus物种之间的系统发育关系。我们的分析显示，新测序的有丝分裂基因组在大小、含量和基因排列方面与迄今为止报道的其他lucanid有丝分裂基因组相当。然而，我们证实了trnS（UCN）和ND1基因之间存在一个较大的基因间隔（IGS），其长度从170 bp（在D. tenuihirsutus）到193 bp（在D. ursulus和D. velutinus）不等。在这一IGS区域内，发现了一个短序列片段（TACTAAATT），该片段在3个种属中都是独一无二的。我们的系统发育分析表明，D. velutinus复合体构成了一个独特的分支，与其他种类的Dorcus sensu stricto有显著的差异。此外，我们重申了原属韩国的D. tenuihirsutus作为一个独立种的有效性，尽管D. ursulus的分类地位有待进一步研究。最后，我们发现大型IGSs的存在和位置对研究鹿角甲虫的进化史和物种划分是有用的。
Mitochondrial genomes of the three lucanid species in the Dorcus velutinus complex - Dorcus velutinus Thomson, D. ursulus Arrow and D. tenuihirsutus Kim and Kim - were assembled and analyzed through next generation sequencing. The mitogenome sequences were used to infer phylogenetic relationships among Dorcus species. Our analyses revealed that the newly sequenced mitogenomes are comparable in their size, content, and gene arrangement to other lucanid mitogenomes reported to date. However, we confirmed the presence of a large intergenic spacer (IGS) between trnS(UCN) and ND1 genes, whose length varied from 170 bp (in D. tenuihirsutus) to 193 bp (in D. ursulus and D. velutinus). Within this IGS region, a short sequence fragment (TACTAAATT) was found uniquely across the three species of Dorcus velutinus complex. Our phylogenetic analyses show that the D. velutinus complex constitutes a distinct clade with a significant divergence from other species of the genus Dorcus sensu stricto. Furthermore, we reaffirm the validity of D. tenuihirsutus - a species originally described from Korea - as a distinct species, though the taxonomic status of D. ursulus remains to be studied further. Finally, we find the presence and location of large IGSs to be useful for studying evolutionary history and species delimitation in stag beetles.