Optimization for beta-mannanase production of a psychrophilic bacterium, Flavobacterium sp.
Optimization for beta-mannanase production of a psychrophilic bacterium, Flavobacterium sp.
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嗜冷细菌黄杆菌属 β-甘露聚糖酶生产的优化。
DOI：
10.1271/bbb.62.655
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
T. Yagi
中科院分区：
文献类型：
--
作者：
M. M. Zakaria;M. Ashiuchi;Shinpei Yamamoto;T. Yagi
我们发现，本研究鉴定的嗜冷细菌黄杆菌在培养基中具有β -甘露聚糖酶（EC 3.2.1.78）活性。甘露聚糖酶在含1.0% （w/v）瓜尔胶（碳源）、0.3% (NH4)2SO4（氮源）和0.06% （w/v）酵母提取物的培养基中活性最高。在4℃条件下培养5天，以单糖或双糖为碳源的培养基中没有发现甘露聚糖酶活性，尽管它们可以被致冷菌利用。只有当细菌在含有多糖的培养基中培养时，才能发现酶的活性。酶制剂在SDS-PAGE洗涤凝胶上显示单个活性带。酶活性的最佳温度为35℃，当反应在10℃下进行时，酶的最佳活性为25%。该-甘露聚糖酶制剂可有效水解瓜尔胶、刺槐豆胶、葡甘露聚糖以及-甘露聚糖。
We found that a psychrophilic bacterium, Flavobacterium sp., characterized in this study, has a beta-mannanase (EC 3.2.1.78) activity in the culture medium. The mannanase activity was the highest in the culture medium, containing 1.0% (w/v) guar gum (as a carbon source), 0.3% (NH4)2SO4 (as a nitrogen source), and 0.06% (w/v) yeast extract, of five-days cultivation at 4 degrees C. No mannanase activity was found in the medium containing a monosaccharide or a disaccharide as a carbon source, although the psychrophile could use them. The enzyme activity was found only when the bacterium was cultured in the medium containing a polysaccharide. The enzyme preparation showed a single activity band on a washed gel of SDS-PAGE. The optimal temperature for the enzyme activity was 35 degrees C. When the reaction was done at 10 degrees C, the enzyme showed 25% of the optimal activity. The beta-mannanase preparation efficiently hydrolyzed guar gum, locust bean gum, and glucomannan as well as beta-mannan.