Endogenous intronic antisense long non-coding RNA, MGAT3-AS1, and kidney transplantation
Endogenous intronic antisense long non-coding RNA, MGAT3-AS1, and kidney transplantation
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内源性内含子反义长非编码RNA、MGAT3-AS1与肾移植
DOI：
10.1038/s41598-019-51409-0
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发表时间：
2019-10-14
影响因子：
4.6
通讯作者：
Tepel, Martin
中科院分区：
文献类型：
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作者：
Nagarajah, Subagini;Xia, Shengqiang;Tepel, Martin
β-1，4-甘露糖蛋白4-β-N-乙酰氨基葡萄糖基转移酶(MGAT3)是天然免疫系统的关键分子。我们验证了内含子反义长非编码RNA MGAT3-AS1可以预测肾移植后延迟移植肾功能的假设。我们前瞻性地评估了129例已故供肾移植受者移植前后的肾功能和MGAT3-AS1。采用定量逆转录聚合酶链式反应检测单个核细胞MGAT3-AS1水平。移植后7天内至少进行一次透析，定义为移植物功能延迟。129例移植受者中有22例发生移植物功能延迟(17%)。移植后移植物功能延迟患者的MGAT3-AS1中位数显著低于移植物即刻功能患者(6.5×10(-6)，IQR 3.0×10(-6)至8.4×10(-6)；vs.8.3×10(-6)，IQR 5.0×10(-6)至12.8×10(-6)；p<；0.05)。移植肾功能延迟的受者术前MGAT3-AS1的中位数(5.1×10(-6)，IQR，2.4×10(-6)~6.8×10(-6))显著低于即刻移植功能受者(8.9×10(-6)，IQR，6.8×10(-6)至13.4×10(-6)；P<；0.05)。受者-操作者特征显示，术前MGAT3-AS1预测移植物功能延迟(曲线下面积为0.83；95%CI为0.65~1.00；p<；0.01)。我们观察到阳性预测值为0.57，阴性预测值为0.95。长的非编码RNA，MGAT3-AS1，预示着已故供体肾移植患者的短期结果。
beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase (MGAT3) is a key molecule for the innate immune system. We tested the hypothesis that intronic antisense long non-coding RNA, MGAT3-AS1, can predict delayed allograft function after kidney transplantation. We prospectively assessed kidney function and MGAT3-AS1 in 129 incident deceased donor kidney transplant recipients before and after transplantation. MGAT3-AS1 levels were measured in mononuclear cells using qRT-PCR. Delayed graft function was defined by at least one dialysis session within 7 days of transplantation. Delayed graft function occurred in 22 out of 129 transplant recipients (17%). Median MGAT3-AS1 after transplantation was significantly lower in patients with delayed graft function compared to patients with immediate graft function (6.5 x 10(-6), IQR 3.0 x 10(-6) to 8.4 x 10(-6) ; vs. 8.3 x 10(-6), IQR 5.0 x 10(-6) to 12.8 x 10(-6); p < 0.05). The median preoperative MGAT3-AS1 was significantly lower in kidney recipients with delayed graft function (5.1 x 10(-6), IQR, 2.4 x 10(-6) to 6.8 x 10(-6)) compared to recipients with immediate graft function (8.9 x 10(-6), IQR, 6.8 x 10(-6) to 13.4 x 10(-6) ; p < 0.05). Receiver-operator characteristics showed that preoperative MGAT3-AS1 predicted delayed graft function (area under curve, 0.83; 95%Cl, 0.65 to 1.00; p < 0.01). We observed a positive predictive value of 0.57, and a negative predictive value of 0.95. Long non-coding RNA, MGAT3-AS1, indicates short-term outcome in patients with deceased donor kidney transplantation.