Genome-scale metabolic modeling of a clostridial co-culture for consolidated bioprocessing
Genome-scale metabolic modeling of a clostridial co-culture for consolidated bioprocessing
复制标题
DOI：
10.1002/biot.201000159
复制
发表时间：
2010-07-01
影响因子：
4.7
通讯作者：
Mahadevan, Radhakrishnan
中科院分区：
文献类型：
--
作者：
Salimi, Fahimeh;Zhuang, Kai;Mahadevan, Radhakrishnan
一种替代的整合生物处理方法是使用含有纤维素分解和产溶剂梭菌的共培养物。已经证明，与C. cellulolyticum，这表明这两个物种之间存在的syntrophy。然而，共培养物中的代谢相互作用还没有很好地理解。为了了解共培养中的代谢相互作用，我们开发了一个基因组规模的代谢模型。解纤维素菌由431个基因、621个反应和603种代谢物组成。梭Cellulolyticum模型可以成功地预测以纤维素为底物的恒化器生长和副产物分泌。然而，在C.在纤维素浓度高于6.7 g/L时，由于缺乏对潜在机制的理解，不能通过动态通量平衡分析来预测。这些纯培养物的基因组规模的代谢模型也被整合使用社区建模框架来开发共培养物中代谢相互作用的动态模型。共培养模拟表明，纤维二糖抑制不能是主要因素，是负责提高纤维素利用相对于单一文化的C。纤维素分解菌
An alternative consolidated bioprocessing approach is the use of a co-culture containing cellulolytic and solventogenic clostridia. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum and Clostridium cellulolyticum is improved compared to the mono-culture of C. cellulolyticum, suggesting the presence of syntrophy between these two species. However, the metabolic interactions in the co-culture are not well understood. To understand the metabolic interactions in the co-culture, we developed a genome-scale metabolic model of C. cellulolyticum comprising of 431 genes, 621 reactions, and 603 metabolites. The C. cellulolyticum model can successfully predict the chemostat growth and byproduct secretion with cellulose as the substrate. However, a growth arrest phenomenon, which occurs in batch cultures of C. cellulolyticum at cellulose concentrations higher than 6.7 g/L, cannot be predicted by dynamic flux balance analysis due to the lack of understanding of the underlying mechanism. These genome-scale metabolic models of the pure cultures have also been integrated using a community modeling framework to develop a dynamic model of metabolic interactions in the co-culture. Co-culture simulations suggest that cellobiose inhibition cannot be the main factor that is responsible for improved cellulose utilization relative to mono-culture of C. cellulolyticum.