The influence of hormones on CD44 expression in endometrial and breast carcinomas.
The influence of hormones on CD44 expression in endometrial and breast carcinomas.
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DOI：
10.3892/or.8.5.987
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发表时间：
2001-09
期刊：
影响因子：
4.2
通讯作者：
B. Durst;Rudiger V. Sorg;Gernot Roder;Beate Betz;M. Beckmann;Dieter Niederacher;H. Bender;Peter Dall
中科院分区：
文献类型：
--
作者：
B. Durst;Rudiger V. Sorg;Gernot Roder;Beate Betz;M. Beckmann;Dieter Niederacher;H. Bender;Peter Dall
细胞表面糖蛋白CD44(CD44v)的不同异构体的表达与啮齿性腺癌细胞的转移潜能和多种类型的人类癌症的预后改变有关。在激素依赖性的妇科癌症中，CD44v的表达模式不同。卵巢类固醇激素及其拮抗剂对CD44v表达的影响目前尚不清楚，目前仅有回溯性相关研究。因此，我们在一项标准化的刺激实验中检测了一些雌激素受体(ER)状态不同的乳腺和子宫内膜癌细胞系中CD44mRNA的表达。与相应的ER阴性细胞系相比，在ER阳性的子宫内膜和乳腺癌细胞系中CD44的总体表达水平更高。选择性剪接异构体的数量和组成与ER的表达状态没有明显的相关性。在所有表达CD44v的细胞系中都检测到三种CD44v亚型，其中两种以前在正常子宫内膜细胞中未见报道。这些异构体可能在这种类型的癌症中具有特定的功能。在本研究的第二部分，研究了(抗)激素对子宫内膜癌细胞株CD44表达的影响。与无胎牛血清(FCS)培养的细胞相比，在含胎牛血清(FCS)的培养液中培养的细胞CD44整体表达增加。雌二醇(1h)对CD44表达有一过性上调作用。子宫内膜癌细胞株RL95-2和Hec-1-A经(抗)激素处理后的CD44剪接图谱显示CD44V亚型如CD44E(CD44v8-V10)的稳定和高表达。在实验期间，只有某些弱表达的亚型改变了它们的表达水平，但没有观察到与激素处理直接相关。综上所述，雌二醇或胎牛血清可增加CD44的整体表达，但卵巢类固醇激素对子宫内膜癌细胞系中CD44v的剪接机制似乎没有直接影响。
The expression of distinct variant isoforms of the cell surface glycoprotein CD44 (CD44v) has been found to be associated with metastatic potential of rodent adenocarcinoma cells and with an altered prognosis in several types of human cancer. In hormone-dependent gynecological cancers, different CD44v expression patterns have been observed. The influence of ovarian steroid hormones and their antagonists on CD44v expression is still unclear, since there are only retrospective correlation studies so far. Therefore, we examined the CD44 mRNA expression in a standardized stimulation experiment in a number of breast and endometrial carcinoma cell lines varying in estrogen receptor (ER) status. Higher CD44 overall expression was observed in ER positive endometrial and breast carcinoma cell lines when compared to corresponding ER negative cell lines. The number and composition of alternatively spliced isoforms showed no clear correlation to the ER expression status. Three CD44v isoforms were detected in all cell lines expressing CD44v, two of which have not been reported previously in normal endometrial cells. These isoforms may have specific functions in this type of carcinoma. In the second part of the study, the influence of (anti-) hormones on CD44 expression in endometrial carcinoma cell lines was examined. CD44 overall expression showed an increase when the cells were grown in medium containing fetal calf serum (FCS) as compared to cells maintained in medium-free of FCS. CD44 expression was transiently increased by estradiol (1 h). The CD44 splice pattern of endometrial cancer cell lines RL95-2 and Hec-1-A, after treatment with (anti-) hormones showed constant and high expression rates for distinct CD44v-isoforms such as CD44E (CD44v8-v10). Only certain weakly expressed isoforms changed their expression level during the experimental period, but no direct correlation to hormone treatment was observed. In conclusion, estradiol or FCS increase CD44 overall expression, but there seems to be no direct influence of ovarian steroid hormones on the CD44v splice machinery in endometrial carcinoma cell lines.